Cellular volatile organic compounds (VOCs) are unique compounds whose metabolic pathways remain enigmatic. To elucidate their metabolism, we investigated the VOCs of lung cancer A549 and 2 non-cancer lung cells (HLB; HBEpC). Neutral sugars and lactate in the medium were measured by colorimetric assay. VOCs were enriched by monotrap and profiled by GC-MS. To investigate the enzymes that change VOC metabolism in cells, we conducted ALDH activity assays and qPCR. ROS (reactive oxygen species) assays were conducted to assess oxidation stress. The colorimetric assay showed that especially A549 and HLB took up sugars from the medium and rapidly secreted lactate into the medium. The VOC profile (GC-MS) revealed a trans-2-hexenol increase, especially in A549 lung cancer cells. This is a novel lipid peroxidation product from animal cells. Based on the absolute quantification data, trans-2-hexenol increased in parallel with number of A549 cancer cells incubated. The qPCR data implies that ADH1c potentially plays an important role in the conversion into trans-2-hexenol.

细胞挥发性有机化合物（VOC）是独特的化合物，其代谢途径仍然是未知的。为了阐明它们的代谢，我们研究了肺癌A549和2种非癌性肺细胞（HLB; HBEpC）的VOC。通过比色法测量培养基中的中性糖和乳酸。通过单阱富集VOC，并通过GC-MS分析。为了研究改变细胞中VOC代谢的酶，我们进行了ALDH活性测定和qPCR。进行了ROS（活性氧）测定以评估氧化应激。比色法显示，尤其是A549和HLB从培养基中吸收糖分，并迅速将乳酸分泌到培养基中。VOC配置文件（GC-MS）显示反式-2-己烯醇增加，尤其是在A549肺癌细胞中。这是来自动物细胞的新型脂质过氧化产物。根据绝对定量数据，反式-2-己烯醇与培养的A549癌细胞数量平行增加。qPCR数据表明ADH1c可能在转化为反式-2-己烯醇。