A growing number of studies point to reduced fertility upon chronic exposure to endocrine-disrupting chemicals (EDCs) such as phthalates and plasticizers. These toxins are ubiquitous and are often found in food and beverage containers, medical devices, as well as in common household and personal care items. Animal studies with EDCs, such as phthalates and bisphenol A have shown a dose-dependent decrease in fertility and embryo toxicity upon chronic exposure. However, limited research has been conducted on the acute effects of these EDCs on male fertility. Here we used a murine model to test the acute effects of four ubiquitous environmental toxins: bisphenol A (BPA), di-2-ethylhexyl phthalate (DEHP), diethyl phthalate (DEP), and dimethyl phthalate (DMP) on sperm fertilizing ability and pre-implantation embryo development. The most potent of these toxins, di-2-ethylhexyl phthalate (DEHP), was further evaluated for its effect on sperm ion channel activity, capacitation status, acrosome reaction and generation of reactive oxygen species (ROS). DEHP demonstrated a profound hazardous effect on sperm fertility by producing an altered capacitation profile, impairing the acrosome reaction, and, interestingly, also increasing ROS production. These results indicate that in addition to its known chronic impact on reproductive potential, DEHP also imposes acute and profound damage to spermatozoa, and thus, represents a significant risk to male fertility.

越来越多的研究表明，长期暴露于邻苯二甲酸盐和增塑剂等破坏内分泌的化学物质（EDC）后，生育力降低。这些毒素无处不在，通常在食品和饮料容器，医疗设备以及常见的家庭和个人护理用品中发现。用EDC（例如邻苯二甲酸酯和双酚A）进行的动物研究显示，长期暴露后，生育能力和胚胎毒性呈剂量依赖性降低。但是，关于这些EDC对男性生育力的急性影响的研究很少。在这里，我们使用鼠模型来测试四种普遍存在的环境毒素：双酚A（BPA），邻苯二甲酸二-2-乙基己基酯（DEHP），邻苯二甲酸二乙酯（DEP）和邻苯二甲酸二甲酯（DMP）对精子受精能力和植入前胚胎发育。进一步评估了这些毒素中最有效的邻苯二甲酸二-2-乙基己基邻苯二甲酸酯（DEHP）对精子离子通道活性，获能状态，顶体反应和活性氧（ROS）产生的影响。DEHP通过改变获能能力，损害顶体反应以及增加ROS的产生，对精子的生育能力产生了深远的危害。这些结果表明，除了已知的对生殖潜能的慢性影响外，DEHP还对精子造成了严重的深刻损害，因此，对男性生育能力构成了重大威胁。DEHP通过改变获能能力，损害顶体反应以及增加ROS的产生，对精子的生育能力产生了深远的危害。这些结果表明，除已知的对生殖潜能的慢性影响外，DEHP还对精子产生了急性和深远的损害，因此，对男性生育能力构成了重大风险。DEHP通过改变获能能力，损害顶体反应以及增加ROS的产生，对精子的生育能力产生了深远的危害。这些结果表明，除已知的对生殖潜能的慢性影响外，DEHP还对精子产生了急性和深远的损害，因此，对男性生育能力构成了重大风险。