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A novel low-cost electrode for recording the local field potential of freely moving rat’s brain
Translational Neuroscience ( IF 2.1 ) Pub Date : 2020-06-05 , DOI: 10.1515/tnsci-2020-0104
Xue-Feng Ding 1 , Yan Gao 1, 2 , Hui Zhang 3 , Yuan Zhang 4 , Shao-Xia Wang 1 , Yong-Qi Zhao 1 , Yi-Zheng Wang 1 , Ming Fan 1
Affiliation  

Local field potentials (LFPs) are involved in almost all cognitive activities of animals. Several kinds of recording electrodes are used for recording LFPs in freely moving animals, including commercial and homemade electrodes. However, commercial recording electrodes are expensive, and their relatively fixed size often causes a steric hindrance effect, especially when combining deep brain stimulation (DBS) with LFP recording, which may not always satisfy the aim of researchers. Currently, an increasing number of researchers are designing their own recording electrodes to lower research costs. Nevertheless, there is no simple universal method to produce low-cost recording electrodes with a specific size according to the target brain area. Thus, we developed a simple method for quickly producing low-cost multiple-channel recording electrodes. To inspect the effectiveness of our self-designed electrode, LFPs were recorded in a Parkinson’s disease (PD) rat model, and an electrical stimulation electrode was implanted into the subthalamic nucleus to verify the space-saving ability of the self-designed recording electrode. The results showed that <30 min was needed to prepare an electrode and that the electrode materials cost <5 dollars. Further investigations showed that our electrode successfully recorded the beta oscillations (12–40 Hz) in the PD rat model. Thus, this method will greatly reduce the cost of recording electrodes and save time for researchers. Additionally, the small size of the electrode will further facilitate DBS research.

中文翻译:

一种新颖的低成本电极,用于记录可自由移动的大鼠大脑的局部场电势

局部场势(LFP)几乎参与了动物的所有认知活动。多种记录电极用于记录自由活动的动物中的LFP,包括商用和自制电极。然而,商用记录电极价格昂贵,并且其相对固定的尺寸通常会引起空间位阻效应,尤其是在将深部脑刺激(DBS)与LFP记录结合使用时,这可能并不总是能满足研究人员的目的。当前,越来越多的研究人员正在设计自己的记录电极以降低研究成本。然而,没有简单的通用方法来根据目标大脑区域生产具有特定尺寸的低成本记录电极。因此,我们开发了一种用于快速生产低成本多通道记录电极的简单方法。为了检查我们自行设计的电极的有效性,在帕金森氏病(PD)大鼠模型中记录了LFP,并将电刺激电极植入到丘脑下核中,以验证自行设计的记录电极的节省空间的能力。结果表明制备电极需要<30分钟,电极材料成本<5美元。进一步的研究表明,我们的电极成功地记录了PD大鼠模型中的β振荡(12–40 Hz)。因此,该方法将大大降低记录电极的成本并为研究人员节省时间。另外,电极的小尺寸将进一步促进DBS研究。将电刺激电极植入丘脑底核中,以验证自行设计的记录电极的节省空间的能力。结果表明制备电极需要<30分钟,电极材料成本<5美元。进一步的研究表明,我们的电极成功地记录了PD大鼠模型中的β振荡(12–40 Hz)。因此,该方法将大大降低记录电极的成本并为研究人员节省时间。另外,电极的小尺寸将进一步促进DBS研究。将电刺激电极植入丘脑底核中,以验证自行设计的记录电极的节省空间的能力。结果表明制备电极需要<30分钟,电极材料成本<5美元。进一步的研究表明,我们的电极成功地记录了PD大鼠模型中的β振荡(12–40 Hz)。因此,该方法将大大降低记录电极的成本并为研究人员节省时间。另外,电极的小尺寸将进一步促进DBS研究。进一步的研究表明,我们的电极成功地记录了PD大鼠模型中的β振荡(12–40 Hz)。因此,该方法将大大降低记录电极的成本并为研究人员节省时间。另外,电极的小尺寸将进一步促进DBS研究。进一步的研究表明,我们的电极成功地记录了PD大鼠模型中的β振荡(12–40 Hz)。因此,该方法将大大降低记录电极的成本并为研究人员节省时间。另外,电极的小尺寸将进一步促进DBS研究。
更新日期:2020-06-30
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