Abstract Nuclear Expressed Sequence Tag (EST) microsatellite markers were developed for the Tasmanian palaeoendemic conifer Lagarostrobos franklinii (Hook.-f.) Quinn for genetic studies. RNAseq data was mined for EST microsatellites, and primer pairs were synthesised from 70 contigs with 50 producing amplification products. Of these 50, 10 reliably amplified and displayed polymorphism across 8 samples representing the entire species range. The genetic diversity of these 10 loci was then examined in three wild populations (84 samples). The number of alleles varied from two to thirteen per locus with the average number of alleles per population ranging between 3.0 – 4.7. Observed and expected heterozygosity ranged from 0.34 – 0.42 and 0.37 – 0.44, respectively. Marker cross-amplification was tested in the New Zealand sister species Manoao colensoi (Hook. f.) Molloy, but no markers amplified reliably, which possibly reflects the age of divergence between these species (~64 million years). These are the first microsatellite markers developed for the monotypic genus Lagarostrobos. They will be valuable for assessing the species extant genetic diversity, the impact of past climatic perturbations and human disturbance and the role of clonal propagation in recruitment.

中文翻译：

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为塔斯马尼亚古地方性针叶树 Lagarostrobos franklinii (Hook. f.) Quinn (Podocarpaceae) 开发 EST 微卫星标记

摘要 核表达序列标签 (EST) 微卫星标记是为塔斯马尼亚古地方性针叶树 Lagarostrobos franklinii (Hook.-f.) Quinn 开发的，用于遗传研究。为 EST 微卫星挖掘 RNAseq 数据，并从 70 个重叠群合成引物对，其中 50 个产生扩增产物。在这 50 个中，10 个在代表整个物种范围的 8 个样本中可靠地扩增并显示了多态性。然后在三个野生种群（84 个样本）中检查了这 10 个基因座的遗传多样性。每个基因座的等位基因数从 2 个到 13 个不等，每个种群的等位基因平均数在 3.0 – 4.7 之间。观察到的和预期的杂合度分别介于 0.34 – 0.42 和 0.37 – 0.44 之间。在新西兰姊妹物种 Manoao colensoi (Hook. f.) Molloy 中测试了标记交叉扩增，但没有可靠地扩增标记，这可能反映了这些物种之间的分化年龄（~6400 万年）。这些是为单型属 Lagarostrobos 开发的第一个微卫星标记。它们对于评估物种现存的遗传多样性、过去气候扰动和人类干扰的影响以及克隆繁殖在招募中的作用非常有价值。