Abstract Three-phase partitioning (TPP) was used to purify plant-esterase from wheat flour. Effect of various process parameters has been evaluated and plant-esterase was purified to 11.35-fold by optimized single step TPP system (50%, (w/v) (NH4)2SO4 saturation, 1:1 (v/v) ratio of crude extract: t-butanol at pH 4).The enzyme was found to be exclusively partitioned in the aqueous phase. Using TPP system, plant-esterase quickly purified to homogeneity with very high purity and activity. On the basis of single factor research, purification process was optimized by using response surface method, established a new type of high efficient purification plant-esterase method. To the best of our knowledge, this is the first report for purification and characterization of plant-esterase by using three phase partitioning (TPP). The results indicated that, TPP is a simple, quick, economical and very attractive process for purification of plant-esterase compared to conventional chromatographic protocols.

中文翻译：

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三相分配法是一种从小麦粉中纯化植物酯酶的快速有效方法

摘要 采用三相分配法(TPP) 纯化小麦粉中的植物酯酶。已评估各种工艺参数的影响，并通过优化的单步 TPP 系统将植物酯酶纯化至 11.35 倍（50%，(w/v) (NH4)2SO4 饱和度，粗品比例为 1:1 (v/v)提取物：pH 4 的叔丁醇。发现该酶仅分配在水相中。使用 TPP 系统，植物酯酶快速纯化至均质，具有极高的纯度和活性。在单因素研究的基础上，采用响应面法优化纯化工艺，建立了一种新型高效纯化植物酯酶法。据我们所知，这是使用三相分配 (TPP) 纯化和表征植物酯酶的第一份报告。结果表明，