We investigated if the PROGINS mutation increases the risk of hepatitis E virus (HEV) infection in liver transplant recipients. PROGINS was analyzed through KASP assay; HEV serologies assessed via enzyme‐linked immunosorbent assay and multiplex cytokine assays were evaluated in plasma with the ProcartaPlex human immunoassay. Seventy liver transplant recipients were evaluated, of which 23 (33%) were HEV immunoglobuln G (IgG)‐positive (HEV+). The frequency of PROGINS in the HEV+ group was 34%, compared with 14% in those that were HEV IgG negative (HEV−). Cytokine measurements in a sub‐set of samples from HEV+/PROGINS+ individuals showed decreased plasma levels of monokine induced by gamma interferon, a proliferation‐inducing ligand, and stem cell factor, as well as increased levels of eotaxin‐3 and interleukin‐31 compared with those HEV−/PROGINS− samples. Our findings suggest an association between the PROGINS mutation and seropositivity for HEV in liver transplant recipients with consequent distorted cytokine levels.

中文翻译：

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黄体酮受体突变与肝移植受者戊型肝炎血清阳性之间的关联。

我们调查了 PROGINS 突变是否会增加肝移植受者感染戊型肝炎病毒 (HEV) 的风险。通过 KASP 分析对 PROGINS 进行分析；通过酶联免疫吸附测定和多重细胞因子测定评估 HEV 血清学，并使用 ProcartaPlex 人类免疫测定在血浆中进行评估。对 70 名肝移植受者进行了评估，其中 23 名 (33%) 为 HEV 免疫球 G (IgG) 阳性 (HEV+)。HEV+ 组的 PROGINS 频率为 34%，而 HEV IgG 阴性 (HEV−) 组的 PROGINS 频率为 14%。HEV+/PROGINS+ 个体样本子集的细胞因子测量显示，与相比，由 γ 干扰素、一种增殖诱导配体和干细胞因子诱导的单核因子血浆水平降低，而嗜酸细胞趋化因子-3 和白细胞介素-31 水平升高。与那些 HEV−/PROGINS− 样本。我们的研究结果表明，肝移植受者中 PROGINS 突变与 HEV 血清阳性之间存在关联，从而导致细胞因子水平扭曲。