The coacervation of collagen hydrolysate (CH) in aqueous solution was triggered by ethanol as poor solvent which has a significant effect on the aggregation and rearrangement of CH macromolecules. In this work, the static coacervation was performed to investigate the influences on coacervates morphology. CH concentration and ethanol temperature had significant influences on coacervates morphology. Treatment for the CH solution of high concentration by lower temperature ethanol (<25°C) tended to form the microspheres morphology. High temperature ethanol was not conducive to CH coacervation, while enhanced the CH solubilization in water‐ethanol. By directly mixing CH solution and ethanol under agitation, dispersed nanospheres could be successfully obtained in suspension. The turbidity and absorbance tests were carried out to further study the CH coacervation behaviors. The stability of milky suspension induced by ethanol was evaluated by a self‐built light transmittance measurement. The result showed that the absorbance and turbidity of CH suspension increased with increasing the V_EtOH ratio and CH concentration. The milky suspension with high V_EtOH would turn clear gradually due to the CH solubilization in water–alcohol. The maximum absorbance presented at pH around 7 where the charges of CH molecules were negative. The coacervation behaviors of CH were very different from that of collagen and gelatin, owing to its lower molecular weight.

中文翻译：

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乙醇引发的胶原水解物凝聚层的结构和稳定性分析

乙醇作为不良溶剂可引发胶原水解产物（CH）的凝聚，这对CH大分子的聚集和重排具有重要影响。在这项工作中，进行静态凝聚研究了对凝聚形态的影响。CH浓度和乙醇温度对凝聚层的形态有重要影响。用较低温度的乙醇（<25°C）处理高浓度的CH溶液倾向于形成微球形态。高温乙醇不利于CH凝聚，而增强了CH在水-乙醇中的溶解度。通过在搅拌下将CH溶液和乙醇直接混合，可以成功地在悬浮液中获得分散的纳米球。进行浊度和吸光度测试以进一步研究CH凝聚行为。乙醇诱导的乳状悬浮液的稳定性通过自建的透光率测量进行了评估。结果表明，CH悬浮液的吸光度和浊度随样品的增加而增加。V _EtOH比和CH浓度。由于CH在水-醇中的溶解作用，具有较高V _EtOH的乳状悬浮液会逐渐变澄清。pH值约为7，CH分子带负电时，出现最大吸光度。CH的凝聚行为由于其较低的分子量而与胶原蛋白和明胶的凝聚行为非常不同。