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A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing.
Trends in Biochemical Sciences ( IF 13.8 ) Pub Date : 2020-06-30 , DOI: 10.1016/j.tibs.2020.06.003
Li Yang 1 , Jia Chen 2
Affiliation  

Two major moieties in genome editing are required for precise genetic changes: the locator moiety for target binding and the effector moiety for genetic engineering. By taking advantage of CRISPR/Cas, which consists of different modules for independent target binding and cleavage, a spectrum of precise and versatile genome editing technologies have been developed for broad applications in biomedical research, biotechnology, and therapeutics. Here, we briefly summarize the progress of genome editing systems from a view of both locator and effector moieties and highlight the advance of newly reported CRISPR-conjugated base editing and prime editing systems. We also underscore distinct mechanisms of off-target effects in CRISPR-conjugated systems and further discuss possible strategies to reduce off-target mutations in the future.



中文翻译:

两个部分的故事:快速发展的基于CRISPR / Cas的基因组编辑。

基因组编辑中的两个主要部分是精确的遗传变化所必需的:用于靶标结合的定位子部分和用于基因工程的效应子部分。通过利用由不同模块组成的CRISPR / Cas进行独立的靶标结合和切割,开发了一系列精确而通用的基因组编辑技术,广泛应用于生物医学研究,生物技术和治疗学。在这里,我们从定位子和效应子部分的角度简要概述了基因组编辑系统的进展,并重点介绍了新报道的CRISPR缀合碱基编辑和主要编辑系统的进展。我们还强调了CRISPR偶联系统中脱靶效应的独特机制,并进一步讨论了减少未来脱靶突变的可能策略。

更新日期:2020-06-30
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