There has been great progress in the ‘green’ chemistry approach for the synthesis of nanoparticles in recent years. Nanoparticles have gained special significance due to their unique properties. Herein we report a bioflocculant facilitated eco-friendly synthesis of copper nanoparticles (CuNPs). The copper nanoparticles were successfully synthesized through the reduction of copper sulphate (3 mM) solution by the bioflocculant (0.5 g). The synthesized nanoparticles were characterized by Scanning electron microscope equipped with elementary detector (SEM), X-ray diffractometer (XRD), Fourier Transform Infrared Spectrophotometer (FT-IR), Transmission electron microscopy (TEM), and UV spectroscope. CuNPs were applied in the treatment of both the domestic and industrial wastewater, removal of dyes and were confirmed for the antimicrobial effect against both Gram-negative and Gram-positive microorganisms. The synthesized nanoparticles showed great potential for industrial application when compared to the chemical flocculant. In comparison to FeCl₃ and bioflocculant, the nanoparticles were found to be effective in BOD and COD removal in coalmine wastewater samples. The highest flocculating activity and removal efficiency was observed at the lowest dosage of 0.2 mg/mL. Moreover, both the Minimal inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) were observed at the concertation of 12.5 μL.

近年来，用于合成纳米颗粒的“绿色”化学方法取得了巨大进展。纳米颗粒由于其独特的性能而具有特殊的意义。本文中，我们报告了一种生物絮凝剂，促进了铜纳米颗粒（CuNPs）的环保合成。通过用生物絮凝剂（0.5 g）还原硫酸铜（3 mM）溶液成功地合成了铜纳米颗粒。合成的纳米颗粒通过配备有元素检测器（SEM）的扫描电子显微镜，X射线衍射仪（XRD），傅立叶变换红外分光光度计（FT-IR），透射电子显微镜（TEM）和紫外光谱仪进行表征。CuNPs被用于处理生活和工业废水，去除染料，并证实对革兰氏阴性和革兰氏阳性微生物均具有抗菌作用。与化学絮凝剂相比，合成的纳米颗粒具有巨大的工业应用潜力。与FeCl相比₃和生物絮凝剂，发现纳米颗粒可有效去除煤矿废水样品中的BOD和COD。在最低剂量为0.2 mg / mL时，观察到了最高的絮凝活性和去除效率。此外，在12.5μL的协同作用下观察到最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。