This work aims to achieve the co-immobilization of laccase and 2,2-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) to improve removal capability of the biocatalyst for pollutants while avoiding potential pollution caused by ABTS. The laccase was immobilized on magnetic chitosan nanoparticles modified with amino-functionalized ionic liquid containing ABTS (MACS-NIL) based on Cu ion chelation (MACS-NIL-Cu-lac). The carrier was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, x-ray diffraction and etc., and electron paramagnetic resonance confirmed the mediator molecule ABTS on the carrier could also play the role of electron transmission. MACS-NIL-Cu-lac presented relatively high immobilization capacity, enhanced activity (1.7-fold that of free laccase), improved pH and temperature adaptability, and increased thermal and storage stability. The removal performance assay found that MACS-NIL-Cu-lac had a good removal efficiency with 100.0 % for 2,4-dichlorophenol in water at 25 °C, even when the concentration reached 50 mg/L. Reusability study showed that after six catalytic runs, the removal efficiency of 2,4-dichlorophenol by MACS-NIL-Cu-lac could still reach 93.2 %. Additionally, MACS-NIL-Cu-lac exhibited higher catalytic efficiencies with 100.0 %, 70.5 % and 93.3 % for bisphenol A, indole, and anthracene, respectively. The high catalytic performance in pure water system obtained by the novel biocatalyst co-immobilizing laccase and electron mediator ABTS showed greater practical application value.

这项工作的目的是实现漆酶和2,2-联胺-二-3-乙基苯并噻唑啉-6-磺酸（ABTS）的共固定化，以提高生物催化剂对污染物的去除能力，同时避免由ABTS引起的潜在污染。将漆酶固定在磁性氨基壳聚糖纳米粒子上，该纳米粒子经基于铜离子螯合的氨基官能化离子液体（含ABTS）（MACS-NIL-Cu-lac）改性。通过傅里叶变换红外光谱，热重分析，X射线衍射等对载体进行了表征，电子顺磁共振证实了载体上的介体分子ABTS也可以起到电子传输的作用。MACS-NIL-Cu-lac具有相对较高的固定能力，增强的活性（是游离漆酶的1.7倍），改善的pH和温度适应性，并提高了热稳定性和存储稳定性。去除性能试验发现，即使浓度达到50 mg / L，MACS-NIL-Cu-lac在25°C下对水中的2,4-二氯苯酚也具有100.0％的良好去除效率。可重复使用性研究表明，经过六次催化运行，MACS-NIL-Cu-lac去除2,4-二氯苯酚的效率仍可达到93.2％。此外，MACS-NIL-Cu-lac表现出更高的催化效率，双酚A，吲哚和蒽的催化效率分别为100.0％，70.5％和93.3％。新型生物催化剂共固定漆酶和电子介体ABTS在纯水体系中具有较高的催化性能，具有较大的实际应用价值。即使在浓度达到50 mg / L时，在25°C的水中也存在4-二氯苯酚。可重复使用性研究表明，经过六次催化运行，MACS-NIL-Cu-lac去除2,4-二氯苯酚的效率仍可达到93.2％。此外，MACS-NIL-Cu-lac表现出更高的催化效率，双酚A，吲哚和蒽的催化效率分别为100.0％，70.5％和93.3％。新型生物催化剂共固定漆酶和电子介体ABTS在纯水体系中具有较高的催化性能，具有较大的实际应用价值。即使在浓度达到50 mg / L时，在25°C的水中也存在4-二氯苯酚。可重复使用性研究表明，经过六次催化运行，MACS-NIL-Cu-lac去除2,4-二氯苯酚的效率仍可达到93.2％。此外，MACS-NIL-Cu-lac表现出更高的催化效率，双酚A，吲哚和蒽的催化效率分别为100.0％，70.5％和93.3％。新型生物催化剂共固定漆酶和电子介体ABTS在纯水体系中具有较高的催化性能，具有较大的实际应用价值。MACS-NIL-Cu-lac表现出更高的催化效率，双酚A，吲哚和蒽的催化效率分别为100.0％，70.5％和93.3％。新型生物催化剂共固定漆酶和电子介体ABTS在纯水体系中具有较高的催化性能，具有较大的实际应用价值。MACS-NIL-Cu-lac表现出更高的催化效率，双酚A，吲哚和蒽的催化效率分别为100.0％，70.5％和93.3％。新型生物催化剂共固定漆酶和电子介体ABTS在纯水体系中具有较高的催化性能，具有较大的实际应用价值。