Aryl-alcohol oxidase (AAO) is a fungal GMC flavoprotein secreted by white-rot fungi that supplies H₂O₂ to the ligninolytic consortium. This enzyme can oxidize a wide array of aromatic alcohols in a highly enantioselective manner, an important trait in organic synthesis. The best strategy to adapt AAO to industrial needs is to engineer its properties by directed evolution, aided by computational analysis. The aim of this review is to describe the strategies and challenges we faced when undertaking laboratory evolution of AAO. After a comprehensive introduction into the structure of AAO, its function and potential applications, the different directed evolution enterprises designed to express the enzyme in an active and soluble form in yeast are described, as well as those to unlock new activities involving the oxidation of secondary aromatic alcohols and the synthesis of furandicarboxylic acids.

芳醇氧化酶（AAO）是由白腐真菌分泌的真菌GMC黄素蛋白，可提供H ₂ O ₂到木质素分解财团。该酶可以高度对映选择性地氧化多种芳香族醇，这是有机合成中的重要特征。使AAO适应工业需求的最佳策略是在计算分析的帮助下，通过定向进化来设计其特性。这篇综述的目的是描述在进行AAO实验室进化时我们面临的策略和挑战。在全面介绍了AAO的结构，其功能和潜在应用后，介绍了设计用于在酵母中以活性和可溶形式表达酶的不同定向进化企业，以及用于解锁涉及次级氧化的新活性的企业。芳香醇和呋喃二甲酸的合成。