In this placebo-controlled, double-blind clinical trial, we have investigated the effect of multi-strain probiotic (Bacillus coagulans Unique IS2, Lactobacillus rhamnosus UBLR58, Bifidobacterium lactis UBBLa70, Lactobacillus plantarum UBLP40 (each of 2 billion CFU); Bifidobacterium breve UBBr01, Bifidobacterium infantis UBBI01 (each of 1 billion CFU)) capsule with glutamine (250 mg) on students facing examination stress. A total of 80 students (18–24 years) were enrolled and randomised to receive multi-strain probiotic or placebo capsules twice a day for 28 days (i.e. pre- and during examination). The stress was analysed at the baseline and the end of the treatment by using the perceived stress scale (PSS), depression anxiety stress scale (DASS), and state-trait anxiety inventory (STAI) questionnaire. The serum cortisol levels were also determined. As a result, at the end of the trial, a total of 74 students completed the study, and those who consumed probiotic capsules showed a significant reduction in PSS, DASS, and STAI scores, and serum cortisol levels from the baseline as compared with placebo. No adverse events were reported during the study. In conclusion, the multi-strain probiotic is effective in reducing stress associated with examination. CTRI/2019/03/018178.

在这项安慰剂对照的双盲临床试验中，我们研究了多菌株益生菌（凝结芽孢杆菌独特IS2，鼠李糖乳杆菌UBLR58，乳酸双歧杆菌UBBLa70，植物乳杆菌UBLP40（每个20亿CFU），短双歧杆菌UBBr01的作用。 ，婴儿双歧杆菌UBBI01（每十亿个CFU）胶囊，含谷氨酰胺（250毫克），用于面对考试压力的学生。共有80名学生（18-24岁）入组并随机分组，每天两次接受多菌株益生菌或安慰剂胶囊，共28天（即检查前和检查中）。通过使用感知压力量表（PSS），抑郁焦虑量表（DASS）和状态-性焦虑量表（STAI）问卷对基线和治疗结束时的压力进行了分析。还测定了血清皮质醇水平。结果，在试验结束时，共有74名学生完成了该研究，与服用安慰剂的人相比，服用益生菌胶囊的人的PSS，DASS和STAI得分以及血清皮质醇水平明显低于安慰剂。 。研究期间未报告不良事件。结论，多菌株益生菌可有效减轻与检查有关的压力。CTRI / 2019/03/018178。