A total of 377 lines from Sorghum Association Panel (SAP) grouped into 10 subpopulations based on eight phenotypic traits, including flag leaf width, terminal branch length, flag leaf height, and flowering time and genetic data were evaluated for grain mold resistance by inoculating them with Alternaria alternata alone, a mixture of A. alternata, Fusarium thapsinum, and Curvularia lunata, and untreated water-sprayed control during 2010, 2013–2015 growing seasons at the Texas A&M AgriLife Research Farm, Burleson County, Texas. Each accession was evaluated at least twice. Eleven accessions exhibited grain mold severity ratings of 2 or less, indicating that they were resistant to highly resistant to the disease when challenged with A. alternata alone, 7 lines identified as such when inoculated with a mixture of A. alternata, F. thapsinum, and C. lunata, and 17 resistant lines were identified under the untreated control. Three lines PI533871, PI576130, and PI656036 exhibited resistant to highly resistant response to grain mold across treatments. When the 10 subpopulations were compared for disease response, accessions within subpopulation 4 had the lowest overall grain mold severity ratings, indicating that this subpopulation (zerazera/caudatum racial type) harbors genes that may contribute to grain mold resistance. A genome-wide association study with over 79,000 single-nucleotide polymorphic (SNP) loci from a publicly available genotype by sequencing dataset available for the (SAP) lines was conducted to identify genomic regions associated with grain mold resistance response based on the individual and combined treatments. In most cases, the top-scoring SNPs were mapped to the nearest or a nearby annotated gene with precedence for a role in host defense.

根据八种表型特征，将来自高粱协会专家组（SAP）的总共377个品系分为10个亚群，这些特征包括旗叶宽度，末端分支长度，旗叶高度和开花时间，并通过接种这些基因来评估其耐谷物霉菌性与格孢单独的混合物，链格孢，尖镰thapsinum，和新月弯孢霉，和未处理的水喷控制在2010年，2013年至2015年在得克萨斯A＆M AgriLife研究农场，伯利森县生长季节。每个登录至少评估两次。11个种质表现出的霉菌严重性等级为2或更低，表明它们在受到挑战后对这种疾病具有高度抵抗力A.孢单独，当与的混合物接种的7行鉴定为这样格孢，˚F。thapsinum，和C.孢，并且在未处理的对照下鉴定出17个抗性系。三行PI533871，PI576130和PI656036在整个处理过程中均表现出对谷物霉菌的高度抗性。当比较这10个亚群的疾病反应时，亚群4内的种质具有最低的整体霉菌严重性等级，表明该亚群（zerazera / caudatum种族类型）带有可能有助于抗霉菌性的基因。进行了全基因组关联研究，通过对（SAP）品系进行测序的数据集，对来自公开可用基因型的超过79,000个单核苷酸多态性（SNP）基因座进行了研究，以基于个体和组合来鉴定与谷物抗霉菌性反应相关的基因组区域治疗。在多数情况下，