The thermo-responsiveness of giant vesicles supporting 2,2,6,6-tetramethylpiperidine (TP), the hindered amine, and its reactivity on the vesicle surface were evaluated with the aim of creating a new artificial biomembrane model using the polymer vesicles having reactive sites on the surface. A light-scattering study demonstrated that the TP-supporting spherical vesicles reversibly self-assembled by responding to temperature changes. The vesicles underwent a two-step disruption by heating, the first, transformation into much smaller and deformed vesicles, and the second, transformation into micelles through the budding separation from the granulated vesicle surface. The vesicles were rapidly restored by cooling due to the aggregation of the micelles. The TP supported on the vesicle surface underwent oxidation by H2O2 to convert into 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), accompanied by transformation from the spherical shape into a sheet. The TEMPO underwent a further reduction by L(+)-ascorbic acid into the hydroxylamine.

中文翻译：

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支持受阻胺作为反应位点的巨型囊泡的热响应性

评估了支持 2,2,6,6-四甲基哌啶 (TP)、受阻胺的巨型囊泡的热响应性及其在囊泡表面的反应性，目的是使用具有反应性的聚合物囊泡创建新的人工生物膜模型。表面上的站点。一项光散射研究表明，支持 TP 的球形囊泡通过响应温度变化可逆地自组装。通过加热，囊泡经历了两步破坏，第一步，转变为更小且变形的囊泡，第二步，通过从颗粒状囊泡表面出芽分离而转化为胶束。由于胶束的聚集，囊泡通过冷却迅速恢复。支持在囊泡表面的 TP 被 H2O2 氧化转化为 2,2,6, 6-四甲基哌啶-1-氧基（TEMPO），伴随着从球形转变为片状。TEMPO 被 L(+)-抗坏血酸进一步还原成羟胺。