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Effects of C-Peptide Replacement Therapy on Bone Microarchitecture Parameters in Streptozotocin-Diabetic Rats.
Calcified Tissue International ( IF 4.2 ) Pub Date : 2020-06-30 , DOI: 10.1007/s00223-020-00716-0
Samantha Maurotti 1 , Cristina Russo 2 , Vincenzo Musolino 3 , Saverio Nucera 3 , Micaela Gliozzi 3 , Miriam Scicchitano 3 , Francesca Bosco 3 , Valeria Maria Morittu 4 , Monica Ragusa 4 , Elisa Mazza 1 , Roberta Pujia 1 , Carmine Gazzaruso 5 , Domenico Britti 4 , Maria Teresa Valenti 6 , Michela Deiana 6, 7 , Stefano Romeo 1, 8 , Sandro Giannini 9 , Luca Dalle Carbonare 6 , Vincenzo Mollace 3 , Arturo Pujia 1 , Tiziana Montalcini 2
Affiliation  

C-peptide therapy protects against diabetic micro- and macrovascular damages and neuropatic complications. However, to date, the role of C-peptide in preventing diabetes-related bone loss has not been investigated. Our aim was to evaluate if C-peptide infusion improves bone quality in diabetic rats. Twenty-three male Wistar rats were randomly divided into three groups: normal control group; sham diabetic control group; diabetic plus C-peptide group. Diabetes was induced by streptozotocin injection and C-peptide was delivered subcutaneously for 6 weeks. We performed micro-CT and histological testing to assess several trabecular microarchitectural parameters. At the end, diabetic plus C-peptide rats had a higher serum C-peptide (p = 0.02) and calcium (p = 0.04) levels and tibia weight (p = 0.02) than the diabetic control group. The diabetic plus C-peptide group showed a higher trabecular thickness and cross-sectional thickness than the diabetic control group (p = 0.01 and p = 0.03). Both the normal control and diabetic plus C-peptide groups had more Runx-2 and PLIN1 positive cells in comparison with the diabetic control group (p = 0.045 and p = 0.034). Diabetic rats receiving C-peptide had higher quality of trabecular bone than diabetic rats not receiving this treatment. If confirmed, C-peptide could have a role in improving bone quality in diabetes.



中文翻译:

C肽替代疗法对链脲佐菌素糖尿病大鼠骨微结构参数的影响。

C肽疗法可预防糖尿病的微血管和大血管损伤以及神经性并发症。然而,迄今为止,尚未研究C肽在预防与糖尿病有关的骨丢失中的作用。我们的目的是评估C肽输注是否可以改善糖尿病大鼠的骨质。将23只雄性Wistar大鼠随机分为三组:正常对照组;和对照组。假糖尿病对照组;糖尿病加C肽组。链脲佐菌素注射液可诱导糖尿病,皮下注射C肽持续6周。我们进行了微CT和组织学测试,以评估一些小梁微结构参数。最后,糖尿病加C肽大鼠的血清C肽(p  = 0.02),钙(p  = 0.04)和胫骨重量(p  = 0.02)。糖尿病加C肽组显示出比糖尿病对照组更高的小梁厚度和横截面厚度(p  = 0.01和p  = 0.03)。与糖尿病对照组相比,正常对照组和糖尿病加C肽组均具有更多的Runx-2和PLIN1阳性细胞(p  = 0.045和p  = 0.034)。与未接受这种治疗的糖尿病大鼠相比,接受C肽的糖尿病大鼠的小梁骨质量更高。如果得到证实,C肽可能会改善糖尿病的骨质。

更新日期:2020-06-30
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