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Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae
Aquaculture International ( IF 2.9 ) Pub Date : 2020-06-29 , DOI: 10.1007/s10499-020-00566-5
Robson Andrade Rodrigues , André Luiz do Nascimento Silva , Mayara Schueroff Siqueira , Fabiana Pilarski , Cassia Rejane Brito Leal , Karin Virgínia Kuibida , Cristiane Meldau de Campos , Carlos Eurico Fernandes

This study evaluated the hematological, biochemical, and histopathological responses of sorubim Pseudoplatystoma spp., experimentally infected with Lactococcus garvieae. Sorubim juveniles (n = 66; 21.3 ± 5.5 g; 17.1 ± 1.3 cm) were infected with L. garvieae by coelmatic inoculation (1.5 × 108 CFU mL−1) and sampling to 1, 3, 7, 14, and 21 days post-inoculation (dpi) for evaluation of hematological, biochemical parameters. Histopathological data of the spleen and kidney were estimated according to the histological alteration index (HAIn). Additionally, a decrease of hematocrit (1, 3, and 14 dpi), hemoglobin, and count of erythrocytes (3, 14, and 21 dpi), blood glucose (3 dpi), and proteinemy (14 dpi) were observed. Monocytes decreased at 7, 14, and 21 dpi; neutrophils at 3, 7, and 21 dpi; and lymphocytes at 21 dpi. Edema, hyperemia, hemorrhage, inflammatory infiltrate, and necrosis were the major histological changes observed in the spleen and kidney. HAIn was higher at 7 to 14 dpi in the spleen. Edema, hyperemia, and hyperplasia with hypertrophy of melanomacrophage centers (MMCs) associated with mild focal necrosis areas, particularly in white pulp, were observed. In the head kidney, it was observed that MMCs and melanomacrophages surrounded the capillaries and dispersed inside the sinusoidal capillaries network. Internalized bacteria by macrophages were commonly observed at 3 to 7 dpi. Tubular neogenesis characterized by nephrogenic bodies and rare MMCs at 21 dpi was observed. The experimental infection with L. garvieae caused significant hematological and biochemical responses. Pathological manifestations are closely related to the spleen and head kidneys mainly represented by hyperplasia and hypertrophy of melanomacrophage centers and mild necrosis areas.



中文翻译:

Sorubim Pseudoplatystoma spp的血液学,生化和组织病理学反应。实验上感染了乳球菌

这项研究评估了实验性感染加维乳杆菌的Sorubim Pseudoplatystoma spp。spp。的血液学,生化和组织病理学反应通过结肠接种(1.5×10 8  CFU mL -1),将Sorubim幼虫(n  = 66; 21.3±5.5 g; 17.1±1.3 cm)感染加维乳杆菌并取样至1,3、7、14和21天接种后(dpi),用于评估血液学,生化指标。根据组织学改变指数(HAI n)估计脾肾的组织病理学数据。)。另外,观察到血细胞比容(1、3和14 dpi),血红蛋白和红细胞计数(3、14和21 dpi),血糖(3 dpi)和蛋白血症(14 dpi)减少。单核细胞在7、14和21 dpi时下降;3、7和21 dpi的中性粒细胞; 和淋巴细胞在21 dpi时。水肿,充血,出血,炎性浸润和坏死是在脾脏和肾脏中观察到的主要组织学变化。HAI ñ脾脏在7到14 dpi时更高。观察到水肿,充血和黑色素巨噬细胞中心(MMCs)肥大,伴有轻度局灶性坏死区域,特别是在白髓中。在头肾中,观察到MMC和黑色巨噬细胞围绕着毛细血管并分散在正弦毛细血管网络内。通常在3至7 dpi观察到巨噬细胞内在的细菌。观察到肾小管新生,其特征是在21 dpi时具有肾原性体和罕见的MMC。garvieae的实验性感染引起了重大的血液和生化反应。病理表现与脾脏和头部肾脏密切相关,主要表现为黑色素巨噬细胞中心和轻度坏死区域的增生和肥大。

更新日期:2020-06-30
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