Solvothermal reactions of Co(NO3)2·6H2O or Ni(NO3)2·6H2O with 1,4-bis((1H-benzo[d]imidazol-1-yl)methyl)benzene (bimb) and 2,5-thiophenedicarboxylic acid (H2tdc) afforded two three-dimensional coordination polymers with the chemical formulas of [Co2(bimb)2(tdc)2]·2H2O (1) and [Ni2(bimb)2(tdc)2]·2H2O (2). Complexes 1-2 were characterized by elemental analysis and single-crystal X-ray diffraction. To evaluate the anti-proliferation ability of the compounds 1 and 2, the Cell counting kit-8 (CCK-8) assay was performed firstly. After that, the Hoechst staining assay was carried out to determine whether the anti-cancer activity of compound has relationship with the cell apoptosis. Next, the western blot detection of the apoptotic proteins was detected to further prove the effect of compound on cells apoptosis.

中文翻译：

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1,4-双（（1H-苯并[d]咪唑-1-基）甲基）苯配体的两种配位聚合物的合成，结构和抗癌活性

Co（NO3）2·6H2O或Ni（NO3）2·6H2O与1,4-双（（1H-苯并[d]咪唑-1-基）甲基）苯）（Bimb）和2,5-噻吩二甲酸的溶剂热反应酸（H2tdc）提供了两种三维配位聚合物，其化学式为[Co2（bimb）2（tdc）2]·2H2O（1）和[Ni2（bimb）2（tdc）2]·2H2O（2）。配合物1-2通过元素分析和单晶X射线衍射表征。为了评估化合物1和2的抗增殖能力，首先进行细胞计数试剂盒8（CCK-8）分析。此后，进行Hoechst染色测定以确定化合物的抗癌活性是否与细胞凋亡有关。接下来，检测凋亡蛋白的蛋白质印迹检测以进一步证明该化合物对细胞凋亡的作用。