BACKGROUND:Pomegranate juice (PJ) contains strong antioxidant polyphenols, and cardiovascular beneficial effects have been reported for the fruit. OBJECTIVE:Using a Langendorff’s model, we hypothesized that PJ has antiarrhythmic effects on isolated rat hearts. METHODS:The hearts in the control group (n = 10) were perfused with Krebs solution. The test groups (n = 10, each) were further perfused with PJ, L-NAME (L), or both (PJL). Following 30 min stabilization, all hearts experienced 30 min global ischemia and 120 min reperfusion. The hearts were monitored for the occurrence of single and salvo arrhythmias, ventricular tachycardia (VT), and ventricular fibrillation (VF). The cardiac release of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and nitrite were measured. In addition, the markers of oxidative stress including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and malondialdehyde (MDA) were assayed in the homogenates of the hearts. RESULTS:The PJ group showed a significant reduction in the occurrence of arrhythmias, an improvement in redox markers, and a rise in nitrite release, compared to the control. Simultaneous treatment with L-NAME significantly reduced, but not fully abolished, these effects. CONCLUSIONS:The current study suggests strong anti-arrhythmic effects for PJ, which are mainly mediated by nitric oxide.

中文翻译：

---

石榴（Punica granatum）的抗心律不齐作用主要由一氧化氮介导

背景：石榴汁（PJ）含有强抗氧化剂多酚，并且据报道对水果有心血管有益作用。目的：使用朗根多夫（Langendorff）模型，我们推测PJ对离体大鼠心脏有抗心律失常作用。方法：对对照组（n = 10）的心脏灌注克雷布斯溶液。测试组（每组n = 10）进一步灌注PJ，L-NAME（L）或两者（PJL）。稳定30分钟后，所有心脏均经历了30分钟的整体缺血和120分钟的再灌注。监测心脏的单发和齐发性心律不齐，室性心动过速（VT）和室性纤颤（VF）的发生。测量了肌酸激酶-MB（CK-MB），乳酸脱氢酶（LDH）和亚硝酸盐的心脏释放。此外，在心脏匀浆中测定了氧化应激的标志物，包括超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GPX），过氧化氢酶（CAT）和丙二醛（MDA）。结果：与对照组相比，PJ组的心律不齐发生率显着降低，氧化还原标志物改善，亚硝酸盐释放增加。用L-NAME同时治疗可显着降低但不能完全消除这些影响。结论：目前的研究表明，PJ具有很强的抗心律失常作用，主要由一氧化氮介导。与对照组相比，亚硝酸盐释放量增加。用L-NAME同时治疗可显着降低但不能完全消除这些影响。结论：目前的研究表明，PJ具有很强的抗心律失常作用，主要由一氧化氮介导。与对照组相比，亚硝酸盐释放量增加。用L-NAME同时治疗可显着降低但不能完全消除这些影响。结论：目前的研究表明，PJ具有很强的抗心律失常作用，主要由一氧化氮介导。