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The Effect of Aqueous Extract of Trachyspermum ammi Seeds and Ibuprofen on Inflammatory Gene Expression in the Cartilage Tissue of Rats with Collagen-Induced Arthritis
Journal of Inflammation Research ( IF 4.5 ) Pub Date : 2020-02-28 , DOI: 10.2147/jir.s236242 Mohsen Korani 1, 2 , Mohammadnabi Jamshidi 2
Journal of Inflammation Research ( IF 4.5 ) Pub Date : 2020-02-28 , DOI: 10.2147/jir.s236242 Mohsen Korani 1, 2 , Mohammadnabi Jamshidi 2
Affiliation
Background and Objectives: Rheumatoid arthritis (RA) is an inflammatory disease treated with nonsteroidal anti–inflammatory drugs that have different side effects. One of the plants used for this purpose in the traditional medicine is Trachyspermum ammi. The present study aimed at investigating the anti–inflammatory effect of this plant on type II collagen-induced arthritis (CIA) in Wistar rats.
Materials and Methods: The study was performed on 35 male Wistar rats. Seven rats were considered as the healthy control group (normal group), and CIA was stablished in the rest. The rats with a model of inflammatory arthritis were divided into four groups. One group did not receive any treatment and three groups were treated orally with ibuprofen (15 mg/kg), aqueous extract of the T. ammi seeds (100 mg/kg), or their combination for 30 days. The effect of different treatments was investigated on the paw thickness, arthritis score, and mRNA level of COX2 and iNOS genes.
Results: CIA increased paw thickness, arthritis score, and COX2 and iNOS mRNA levels compared to those of the normal group. Treatment with ibuprofen and aqueous extract alone or in combination reduced the studied variables. Reduction in the paw thickness, arthritis score, and iNOS mRNA level was more in the ibuprofen-treated group than the T. ammi extract-treated group, but treatment with T. ammi extract reduced COX2 mRNA level more than ibuprofen.
Conclusion: It seems that the aqueous extract of T. ammi can be used alone or in combination with ibuprofen to treat RA.
Keywords: rheumatoid arthritis, Trachyspermum ammi, ibuprofen, cyclooxygenase-2, nitric oxide synthase type II
中文翻译：
Trachyspermum ammi 种子水提物和布洛芬对胶原性关节炎大鼠软骨组织炎症基因表达的影响
背景和目的：类风湿关节炎（RA）是一种炎症性疾病，使用具有不同副作用的非甾体抗炎药治疗。传统医学中用于此目的的植物之一是Trachyspermum ammi。本研究旨在研究这种植物对 Wistar 大鼠 II 型胶原诱导性关节炎 (CIA) 的抗炎作用。
材料和方法：该研究是在 35 只雄性 Wistar 大鼠上进行的。7只大鼠作为健康对照组（正常组），其余均设置CIA。将具有炎性关节炎模型的大鼠分为四组。一组未接受任何治疗，三组口服布洛芬 (15 mg/kg)、T. ammi种子的水提取物 (100 mg/kg) 或它们的组合，持续 30 天。研究了不同治疗对爪子厚度、关节炎评分以及COX2和iNOS基因 mRNA 水平的影响。
结果： CIA 增加了爪子厚度、关节炎评分以及COX2和iNOSmRNA水平与正常组比较。单独或联合使用布洛芬和水提取物治疗减少了研究变量。布洛芬治疗组的爪厚度、关节炎评分和iNOS mRNA 水平的降低比T. ammi提取物治疗组更多，但T. ammi提取物治疗比布洛芬更能降低COX2 mRNA 水平。
结论：似乎T. ammi的水提物可以单独使用或与布洛芬联合使用来治疗RA。
关键词：类风湿性关节炎，Trachyspermum ammi，布洛芬，环氧合酶-2，一氧化氮合酶II型
更新日期：2020-02-28
Materials and Methods: The study was performed on 35 male Wistar rats. Seven rats were considered as the healthy control group (normal group), and CIA was stablished in the rest. The rats with a model of inflammatory arthritis were divided into four groups. One group did not receive any treatment and three groups were treated orally with ibuprofen (15 mg/kg), aqueous extract of the T. ammi seeds (100 mg/kg), or their combination for 30 days. The effect of different treatments was investigated on the paw thickness, arthritis score, and mRNA level of COX2 and iNOS genes.
Results: CIA increased paw thickness, arthritis score, and COX2 and iNOS mRNA levels compared to those of the normal group. Treatment with ibuprofen and aqueous extract alone or in combination reduced the studied variables. Reduction in the paw thickness, arthritis score, and iNOS mRNA level was more in the ibuprofen-treated group than the T. ammi extract-treated group, but treatment with T. ammi extract reduced COX2 mRNA level more than ibuprofen.
Conclusion: It seems that the aqueous extract of T. ammi can be used alone or in combination with ibuprofen to treat RA.
Keywords: rheumatoid arthritis, Trachyspermum ammi, ibuprofen, cyclooxygenase-2, nitric oxide synthase type II
中文翻译：
Trachyspermum ammi 种子水提物和布洛芬对胶原性关节炎大鼠软骨组织炎症基因表达的影响
背景和目的：类风湿关节炎（RA）是一种炎症性疾病，使用具有不同副作用的非甾体抗炎药治疗。传统医学中用于此目的的植物之一是Trachyspermum ammi。本研究旨在研究这种植物对 Wistar 大鼠 II 型胶原诱导性关节炎 (CIA) 的抗炎作用。
材料和方法：该研究是在 35 只雄性 Wistar 大鼠上进行的。7只大鼠作为健康对照组（正常组），其余均设置CIA。将具有炎性关节炎模型的大鼠分为四组。一组未接受任何治疗，三组口服布洛芬 (15 mg/kg)、T. ammi种子的水提取物 (100 mg/kg) 或它们的组合，持续 30 天。研究了不同治疗对爪子厚度、关节炎评分以及COX2和iNOS基因 mRNA 水平的影响。
结果： CIA 增加了爪子厚度、关节炎评分以及COX2和iNOSmRNA水平与正常组比较。单独或联合使用布洛芬和水提取物治疗减少了研究变量。布洛芬治疗组的爪厚度、关节炎评分和iNOS mRNA 水平的降低比T. ammi提取物治疗组更多，但T. ammi提取物治疗比布洛芬更能降低COX2 mRNA 水平。
结论：似乎T. ammi的水提物可以单独使用或与布洛芬联合使用来治疗RA。
关键词：类风湿性关节炎，Trachyspermum ammi，布洛芬，环氧合酶-2，一氧化氮合酶II型
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