Introduction: A correlation is established between the efficacy of Chinese herbal medicine and its charcoal drugs. Lonicerae japonicae Flos (LJF) is commonly used to treat fever, carbuncle, and tumors, among others. LJF Carbonisatas (LJFC) is preferred for detoxifying and relieving dysentery and its related symptoms. However, the mechanisms underlying the effects of LJFC remain unknown.
Aim: The aim of this study was to explore the effects of LJFC-derived carbon dots (LJFC-CDs) on lipopolysaccharide (LPS)-induced fever and hypothermia rat models.
Methods: LJFC-CDs were characterized using transmission electron microscopy, high-resolution transmission electron microscopy, Fourier-transform infrared, ultraviolet, fluorescence, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. The anti-inflammatory effects of LJFC-CDs were evaluated and confirmed using rat models of LPS-induced fever or hypothermia.
Results: The LJFC-CDs ranged from 1.0 to 10.0 nm in diameter, with a yield of 0.5%. LJFC-CDs alleviated LPS-induced inflammation, as demonstrated by the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 and the recovery of normal body temperature.
Conclusion: LJFC-CDs may have an anti-inflammatory effect and a potential to alleviate fever and hypothermia caused by inflammation.

Keywords: carbon dots, Lonicerae japonicae Flos, anti-inflammatory, lipopolysaccharide


中文翻译：

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金银花碳点衍生碳点对脂多糖诱导的大鼠发热和低温模型的影响。

引言：中草药的功效与其炭药之间存在相关性。Lonicerae japonicae Flos (LJF) 通常用于治疗发热、痈肿和肿瘤等。LJF Carbonisatas (LJFC) 是首选用于解毒和缓解痢疾及其相关症状。然而，LJFC 影响的潜在机制仍然未知。
目的：本研究旨在探讨 LJFC 衍生碳点 (LJFC-CDs) 对脂多糖 (LPS) 诱导的发热和低温大鼠模型的影响。
方法：使用透射电子显微镜、高分辨率透射电子显微镜、傅里叶变换红外、紫外、荧光、X 射线光电子能谱、X 射线衍射和高效液相色谱对 LJFC-CD 进行了表征。使用 LPS 诱导的发热或体温过低的大鼠模型评估和确认 LJFC-CD 的抗炎作用。
结果： LJFC-CDs 的直径范围为 1.0 到 10.0 nm，产率为 0.5%。LJFC-CDs 减轻了 LPS 诱导的炎症，这可以通过肿瘤坏死因子-α、白细胞介素 (IL)-1β 和 IL-6 的表达以及正常体温的恢复来证明。
结论： LJFC-CDs可能具有抗炎作用，并有可能减轻炎症引起的发热和体温过低。

关键词：碳点，金银花，抗炎，脂多糖