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Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice.
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-05-29 , DOI: 10.2147/ijn.s249942 Zohre Sadeghi 1 , Mahdi Fasihi-Ramandi 2 , Saeid Bouzari 1
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-05-29 , DOI: 10.2147/ijn.s249942 Zohre Sadeghi 1 , Mahdi Fasihi-Ramandi 2 , Saeid Bouzari 1
Affiliation
Introduction: Vaccine formulation with appropriate adjuvants is an attractive approach to develop protective immunity against pathogens. Calcium phosphate nanoparticles (CaPNs) are considered as ideal adjuvants and delivery systems because of their great potential for enhancing immune responses. In the current study, we have designed nanoparticle-based vaccine candidates to induce immune responses and protection against B. melitensis and B. abortus.
Materials and Methods: For this purpose, we used three Brucella antigens (FliC, 7α-HSDH, BhuA) and two multi-epitopes (poly B and poly T) absorbed by CaPNs. The efficacy of each formulation was evaluated by measuring humoral, cellular and protective responses in immunized mice.
Results: The CaPNs showed an average size of about 90 nm with spherical shape and smooth surface. The CaPNs-adsorbed proteins displayed significant increase in cellular and humoral immune responses compared to the control groups. In addition, our results showed increased ratio of specific IgG2a (associated with Th1) to specific IgG1 (associated with Th2). Also, immunized mice with different vaccine candidate formulations were protected against B. melitensis 16M and B. abortus 544, and showed same levels of protection as commercial vaccines (B. melitensis Rev.1 and B. abortus RB51) except for BhuA-CaPNs.
Discussion: Our data support the hypothesis that these antigens absorbed with CaPNs could be effective vaccine candidates against B. melitensis and B. abortus.
Keywords: calcium phosphate nanoparticle, adjuvant, Brucella, vaccine, immune response
中文翻译：
基于纳米粒子的布鲁氏菌病疫苗：磷酸钙纳米粒子吸附的抗原诱导小鼠的交叉保护反应。
简介：含有适当佐剂的疫苗配方是一种有吸引力的方法来开发针对病原体的保护性免疫。磷酸钙纳米颗粒 (CaPNs) 被认为是理想的佐剂和递送系统，因为它们具有增强免疫反应的巨大潜力。在目前的研究中，我们设计了基于纳米粒子的候选疫苗，以诱导免疫反应并保护B. melitensis和B. abortus。
材料和方法：为此，我们使用了三个布鲁氏菌抗原（FliC、7α-HSDH、BhuA）和被 CaPNs 吸收的两个多表位（poly B 和 poly T）。通过测量免疫小鼠的体液、细胞和保护反应来评估每种制剂的功效。
结果： CaPNs的平均尺寸约为90 nm，呈球形，表面光滑。与对照组相比，CaPNs 吸附的蛋白质显示出细胞和体液免疫反应的显着增加。此外，我们的结果显示特异性 IgG2a（与 Th1 相关）与特异性 IgG1（与 Th2 相关）的比例增加。此外，使用不同候选疫苗配方的免疫小鼠对B. melitensis 16M 和B. abortus 544 具有保护作用，并显示出与商业疫苗相同的保护水平。B. melitensis Rev.1 和B. abortus RB51)，BhuA-CaPN 除外。
讨论：我们的数据支持这样的假设，即这些被 CaPNs 吸收的抗原可能是针对B. melitensis和B. abortus 的有效候选疫苗。
关键词：磷酸钙纳米颗粒，佐剂，布鲁氏菌，疫苗，免疫反应
更新日期：2020-05-29
Materials and Methods: For this purpose, we used three Brucella antigens (FliC, 7α-HSDH, BhuA) and two multi-epitopes (poly B and poly T) absorbed by CaPNs. The efficacy of each formulation was evaluated by measuring humoral, cellular and protective responses in immunized mice.
Results: The CaPNs showed an average size of about 90 nm with spherical shape and smooth surface. The CaPNs-adsorbed proteins displayed significant increase in cellular and humoral immune responses compared to the control groups. In addition, our results showed increased ratio of specific IgG2a (associated with Th1) to specific IgG1 (associated with Th2). Also, immunized mice with different vaccine candidate formulations were protected against B. melitensis 16M and B. abortus 544, and showed same levels of protection as commercial vaccines (B. melitensis Rev.1 and B. abortus RB51) except for BhuA-CaPNs.
Discussion: Our data support the hypothesis that these antigens absorbed with CaPNs could be effective vaccine candidates against B. melitensis and B. abortus.
Keywords: calcium phosphate nanoparticle, adjuvant, Brucella, vaccine, immune response
中文翻译：
基于纳米粒子的布鲁氏菌病疫苗：磷酸钙纳米粒子吸附的抗原诱导小鼠的交叉保护反应。
简介：含有适当佐剂的疫苗配方是一种有吸引力的方法来开发针对病原体的保护性免疫。磷酸钙纳米颗粒 (CaPNs) 被认为是理想的佐剂和递送系统，因为它们具有增强免疫反应的巨大潜力。在目前的研究中，我们设计了基于纳米粒子的候选疫苗，以诱导免疫反应并保护B. melitensis和B. abortus。
材料和方法：为此，我们使用了三个布鲁氏菌抗原（FliC、7α-HSDH、BhuA）和被 CaPNs 吸收的两个多表位（poly B 和 poly T）。通过测量免疫小鼠的体液、细胞和保护反应来评估每种制剂的功效。
结果： CaPNs的平均尺寸约为90 nm，呈球形，表面光滑。与对照组相比，CaPNs 吸附的蛋白质显示出细胞和体液免疫反应的显着增加。此外，我们的结果显示特异性 IgG2a（与 Th1 相关）与特异性 IgG1（与 Th2 相关）的比例增加。此外，使用不同候选疫苗配方的免疫小鼠对B. melitensis 16M 和B. abortus 544 具有保护作用，并显示出与商业疫苗相同的保护水平。B. melitensis Rev.1 和B. abortus RB51)，BhuA-CaPN 除外。
讨论：我们的数据支持这样的假设，即这些被 CaPNs 吸收的抗原可能是针对B. melitensis和B. abortus 的有效候选疫苗。
关键词：磷酸钙纳米颗粒，佐剂，布鲁氏菌，疫苗，免疫反应
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