Interest in novel delivery systems that improve the cytoprotective and antioxidant effects of natural drugs has been explored recently due to the increase in the incidence of chronic diseases in which oxidation mechanisms are involved. Curcumin is a phenolic compound recently shown to be clinically significant due to its anti-inflammatory, anticancer, and antioxidant properties. However, this molecule possesses a low bioavailability and a high degradation rate in the presence of light. Therefore, we prepared nanoparticles of poly-ε-caprolactone and Pluronic® F-68 as a stabilizer and loaded these with curcumin (Cur–PCL nanoparticles) for antioxidant and cytoprotective applications. The nanoparticles did not induce cell death, but they did reduce cell proliferation without affecting cell migration and cell adhesion. Interestingly, Cur–PCL and poly-ε-caprolactone nanoparticles reduced the oxidative stress induced by hydrogen peroxide and presented a cytoprotective effect. Remarkably, poly-ε-caprolactone nanoparticles showed a decrement of 30% in reactive oxygen species presence compared to the positive control. The decrease of reactive oxygen species derived from the administration of poly-ε-caprolactone nanoparticles could be attributed to the presence of Pluronic® F-68. Taken together, these data indicated that these nanoparticles might have a clinical application in disorders related to reactive oxygen species formation.

中文翻译：

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负载姜黄素的聚-ε-己内酯纳米颗粒在活性氧存在下表现出抗氧化和细胞保护作用

由于涉及氧化机制的慢性疾病的发病率增加，最近人们对改善天然药物的细胞保护和抗氧化作用的新型递送系统产生了兴趣。姜黄素是一种酚类化合物，由于其抗炎、抗癌和抗氧化特性，最近被证明具有临床意义。然而，该分子在光存在下具有低生物利用度和高降解率。因此，我们制备了聚-ε-己内酯和 Pluronic® F-68 纳米颗粒作为稳定剂，并在其中加入姜黄素（Cur-PCL 纳米颗粒）用于抗氧化和细胞保护应用。纳米颗粒不会诱导细胞死亡，但它们确实减少了细胞增殖而不影响细胞迁移和细胞粘附。有趣的是，Cur-PCL 和聚-ε-己内酯纳米颗粒降低了过氧化氢诱导的氧化应激，并具有细胞保护作用。值得注意的是，与阳性对照相比，聚-ε-己内酯纳米颗粒的活性氧含量减少了 30%。聚-ε-己内酯纳米颗粒给药导致的活性氧种类的减少可归因于 Pluronic® F-68 的存在。总之，这些数据表明这些纳米颗粒可能在与活性氧形成相关的疾病中具有临床应用。与阳性对照相比，聚-ε-己内酯纳米颗粒在活性氧的存在下显示出 30% 的减少。聚-ε-己内酯纳米颗粒给药导致的活性氧种类的减少可归因于 Pluronic® F-68 的存在。总之，这些数据表明这些纳米颗粒可能在与活性氧形成相关的疾病中具有临床应用。与阳性对照相比，聚-ε-己内酯纳米颗粒在活性氧的存在下显示出 30% 的减少。聚-ε-己内酯纳米颗粒给药导致的活性氧种类的减少可归因于 Pluronic® F-68 的存在。总之，这些数据表明这些纳米颗粒可能在与活性氧形成相关的疾病中具有临床应用。