In this study, we aimed at fabricating a novel porous physical construct from quince seed mucilage for translational medicine applications. To achieve this goal, quince seed mucilage was extracted, molded, and freeze-dried. After being freeze-dried, the molded constructs were chemically crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide to maintain the mechanical integrity of the structure. The fabricated scaffolds were characterized in-depth by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller analysis, thermogravimetric analysis, and dynamic mechanical analysis in addition to the swelling, liquid uptake, and porosity tests. The extraction yield of mucilage was calculated to be 6.28% ± 0.40% (n = 3). The swelling ratio of crosslinked quince seed mucilage–derived scaffolds was found to be 12,677.50% ± 388.82% (n = 3), whereas the porosity of crosslinked quince seed mucilage–derived scaffolds was 83.43% ± 2.84% (n = 3). The analyses confirmed the crosslinked quince seed mucilage–derived scaffolds to be possessed interconnected, highly porous structure. Afterward, human adipose-derived mesenchymal stem cells were seeded on the crosslinked quince seed mucilage–derived scaffolds, and the cell viability on the scaffolds was assessed with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The MTT results revealed the scaffolds not to be possessed any cytotoxic effect on seeded cells. Human adipose-derived mesenchymal stem cells adhesion and migration on the crosslinked quince seed mucilage–derived scaffolds were also evaluated histologically using hematoxylin and eosin staining in addition to scanning electron microscopy analysis. In conclusion, we believe that crosslinked quince seed mucilage–derived scaffolds have the potential to be an alternative to routinely used polysaccharides in regenerative medicine applications.

中文翻译：

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用于人类脂肪源间充质干细胞的木瓜种子粘液的生物工程三维物理结构

在这项研究中，我们旨在从木瓜​​种子粘液中制造一种用于转化医学应用的新型多孔物理结构。为了实现这一目标，木瓜种子粘液被提取、成型和冷冻干燥。冷冻干燥后，模制结构与 1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺/N-羟基琥珀酰亚胺进行化学交联，以保持结构的机械完整性。除了溶胀、液体吸收和孔隙率测试外，还通过扫描电子显微镜、傅里叶变换红外光谱、Brunauer-Emmett-Teller 分析、热重分析和动态力学分析对制造的支架进行了深入表征。粘液的提取率计算为 6.28% ± 0.40% (n = 3)。交联木瓜籽粘液衍生支架的溶胀率为 12,677.50% ± 388.82% (n = 3)，而交联木瓜籽粘液衍生支架的孔隙率为 83.43% ± 2.84% (n = 3)。分析证实交联木瓜种子粘液衍生的支架具有相互连接的高度多孔结构。然后，将人脂肪来源的间充质干细胞接种在交联木瓜种子粘液来源的支架上，并用 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四唑评估支架上的细胞活力溴化物 (MTT) 测定。MTT 结果表明支架对接种的细胞不具有任何细胞毒性作用。除了扫描电子显微镜分析外，还使用苏木精和伊红染色在组织学上评估了人脂肪来源间充质干细胞在交联木瓜种子粘液来源支架上的粘附和迁移。总之，我们相信交联木瓜种子粘液衍生支架有可能成为再生医学应用中常规使用的多糖的替代品。