Periodontitis is a chronic inflammatory disease that causes gum tissue degeneration and alveolar bone and tooth loss. The aim of this study is to develop a multifunctional matrix for the treatment of periodontitis and enhancement of regeneration of the periodontal tissue. The matrix was prepared from vitamin E containing hydrogel made of alginate and gelatin, and doxycycline HCl containing methoxy poly(ethylene glycol)-block-polycaprolactone micelles. Methoxy poly(ethylene glycol)-block-polycaprolactone was synthesized with ring-opening polymerization technique and characterized by proton nuclear magnetic resonance (1H NMR), Fourier-transform infrared spectroscopy, differential scanning calorimetry, and gel permeation chromatography. Micelles were characterized by measuring zeta potential, hydrodynamic diameter, drug encapsulation efficiency, drug loading capacity, and in vitro drug-release kinetics. Micelles were obtained with an average size of 164 nm and drug loading amount of 5.8%. The activity of doxycycline HCl–loaded micelles and vitamin E containing hydrogels was determined against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with disk diffusion method. Bio-efficacy of micelle-loaded alginate–gelatin hydrogels were tested in vitro using L929 fibroblasts and dental pulp stem cells. Doxycycline HCl–loaded micelles and vitamin E containing hydrogels showed a sustained release and exhibited inhibition zone against E. coli and S. aureus. Hydrogels with vitamin E and doxycycline HCl–loaded micelles promoted osteogenic differentiation of dental pulp stem cells. Results suggest that alginate–gelatin hydrogels containing doxycycline HCl–loaded micelles and vitamin E can be good candidates for the treatment of periodontitis and tissue regeneration.

中文翻译：

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用于治疗和再生目的的多功能牙周膜

牙周炎是一种慢性炎症性疾病，会导致牙龈组织退化以及牙槽骨和牙齿脱落。本研究的目的是开发一种用于治疗牙周炎和促进牙周组织再生的多功能基质。基质由含有维生素 E 的藻酸盐和明胶制成的水凝胶和含有甲氧基聚（乙二醇）-嵌段-聚己内酯胶束的盐酸多西环素制备。甲氧基聚（乙二醇）-嵌段-聚己内酯采用开环聚合技术合成，并通过质子核磁共振（1H NMR）、傅里叶变换红外光谱、差示扫描量热法和凝胶渗透色谱法表征。胶束通过测量 zeta 电位、流体动力学直径、药物包封效率、载药量和体外药物释放动力学。获得平均粒径为164 nm、载药量为5.8%的胶束。用圆盘扩散法测定了载有盐酸多西环素的胶束和含有维生素 E 的水凝胶对大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）的活性。使用 L929 成纤维细胞和牙髓干细胞在体外测试了装载胶束的藻酸盐-明胶水凝胶的生物功效。负载盐酸多西环素的胶束和含有维生素 E 的水凝胶显示出持续释放并表现出对大肠杆菌和金黄色葡萄球菌的抑制区。含有维生素 E 和盐酸多西环素胶束的水凝胶促进牙髓干细胞的成骨分化。