In this study, films of O-carboxymethyl chitosan with Mimosa tenuiflora extract were manufactured, characterized, and evaluated. In this work, both the synthesis of O-carboxymethyl chitosan and the extraction of the active ingredient of Mimosa tenuiflora extract from the cortex are described. First, the extract of Mimosa tenuiflora in water was obtained by precipitation with ethanol, filtering, and concentrating. Subsequently, a study was conducted of scratch wound healing to determine the optimal concentration of extract to be used in the manufacture of films. The produced O-carboxymethyl chitosan films and the Mimosa tenuiflora extract were mixed, and their chemical composition, tensile properties, and wettability were characterized by Fourier-transform infrared spectroscopy, mechanical tests, and contact angle measurement. The antimicrobial properties of the films were tested by turbidimetry using two types of bacteria. In addition, a study of the enzymatic degradation of the films with the enzyme lysozyme was performed. Finally, in vitro studies to assess the biocompatibility and cytotoxicity of films with fibroblastic cells were carried out as well as the kinetic analysis of healing in mice. It was found that the addition of Mimosa tenuiflora extract in the polymer matrix of the films made with O-carboxymethyl chitosan improves the proliferation of fibroblast and accelerates wound healing, thus providing a novel biomaterial for skin regeneration.

中文翻译：

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含含羞草提取物用于皮肤再生和伤口愈合的新型 O-羧甲基壳聚糖膜的表征和评价

在本研究中，制备、表征和评估了含含羞草提取物的 O-羧甲基壳聚糖薄膜。在这项工作中，描述了 O-羧甲基壳聚糖的合成和从皮层中提取含羞草提取物的活性成分。首先，用乙醇沉淀、过滤、浓缩得到含羞草的水中提取物。随后，对划痕伤口愈合进行了研究，以确定用于制造薄膜的提取物的最佳浓度。将制得的O-羧甲基壳聚糖薄膜与含羞草提取物混合，通过傅里叶变换红外光谱、力学测试和接触角测量对其化学成分、拉伸性能和润湿性进行表征。使用两种细菌通过比浊法测试薄膜的抗菌性能。此外，还进行了溶菌酶对薄膜的酶促降解的研究。最后，进行了体外研究，以评估具有成纤维细胞的薄膜的生物相容性和细胞毒性，以及小鼠愈合的动力学分析。研究发现，在O-羧甲基壳聚糖制成的薄膜的聚合物基质中加入含羞草提取物，可促进成纤维细胞的增殖并加速伤口愈合，从而为皮肤再生提供了一种新型生物材料。进行了体外研究，以评估具有成纤维细胞的薄膜的生物相容性和细胞毒性，以及小鼠愈合的动力学分析。研究发现，在O-羧甲基壳聚糖制成的薄膜的聚合物基质中加入含羞草提取物，可促进成纤维细胞的增殖并加速伤口愈合，从而为皮肤再生提供了一种新型生物材料。进行了体外研究，以评估具有成纤维细胞的薄膜的生物相容性和细胞毒性，以及小鼠愈合的动力学分析。研究发现，在O-羧甲基壳聚糖制成的薄膜的聚合物基质中加入含羞草提取物，可促进成纤维细胞的增殖并加速伤口愈合，从而为皮肤再生提供了一种新型生物材料。