Four cpt 1 genes (cpt 1α1a, cpt 1α2a, cpt 1α2b, and cpt 1β) were identified in the Nile tilapia genome. Two transmembrane helix domains (TMH) were identified for Cpt 1α1a, Cpt 1α2a, and Cpt 1β, while Cpt 1α2b had only one TMH domain. Evidence was found of conserved gene synteny between cpt 1 genes from Nile tilapia and the cpt 1/CPT 1 genes of zebrafish and human. Phylogenetic analysis showed that Nile tilapia Cpt 1 sequences clustered in distinct clades with their orthologous Cpt 1/CPT 1 from other vertebrates. Nile tilapia cpt 1α1a, cpt 1α2a, cpt 1α2b, and cpt 1β contain 18 coding exons encoding polypeptides of 771, 784, 788, and 786 amino acids in length, respectively. The cpt 1 genes were determined in all the tested tissues with varying tissue distribution patterns. These findings suggest that (1) cpt 1α1a, cpt 1α2a, and cpt 1α2b arose in the Nile tilapia genome as a result of the teleost-specific whole-genome duplication; (2) nonfunctionalization is the most likely cause of the loss of cpt 1α1b in the Nile tilapia genome; (3) the different tissue-specific transcription of cpt 1α2a and cpt 1α2b may be either due to the sub- or the neo-functionalization of transcriptional control side.

四CPT 1个基因（CPT1α1a，CPT1α2a，CPT1α2b和CPT1β）在尼罗河鉴定罗非鱼的基因组。为Cpt1α1a，Cpt1α2a和Cpt1β鉴定了两个跨膜螺旋结构域（TMH），而Cpt1α2b仅具有一个TMH结构域。证据之间发现保守的基因同线性的CPT 1个从尼罗罗非鱼基因和CPT 1 / CPT 1个基因斑马鱼和人的。系统发育分析表明，尼罗罗非鱼Cpt 1序列与其他脊椎动物的直系同源Cpt 1 / CPT 1聚集在不同的进化枝中。尼罗罗非鱼CPT1α1a，CPT1α2a，CPT1α2b和CPT1β含有18个编码外显子，分别编码长度为771、784、788和786个氨基酸的多肽。的CPT 1倍中的所有具有不同的组织分布模式的测试组织中确定的基因。这些发现表明：（1）硬骨鱼特异性全基因组复制的结果是尼罗罗非鱼基因组中出现了cpt1α1a，cpt1α2a和cpt1α2b；（2）非功能化是尼罗罗非鱼基因组中cpt1α1b丢失的最可能原因；（3）cpt1α2a和cpt1α2b不同的组织特异性转录可能是由于转录控制侧的亚功能化或新功能化。