Background Even though the widespread of nanoalumina and their benefits in all fields, its potential impacts on male reproductive system have limited information. Objective The present study was conducted to investigate the testicular dysfunction of nanoalumina and the protective role of pumpkin seed oil (PSO) against potential adverse impacts induced by alumina nanoparticles (Al 2 O 3 -NPs) in male rat. Methodology Al 2 O 3 -NPs were administered to the rat orally at a dose of 70 mg/kg body weight once a day for 28 successive days, while pumpkin seed oil was administered to the rat orally at 4 mL/kg b w before administration of Al 2 O 3 -NPs, once a day for 28 successive days. After the administration period, sperm concentration, motility, morphology, and DNA damage, as biomarkers of reproductive toxic effects, were evaluated using sperm analysis and comet assays, and histopathological examination of testis was performed. In addition, level of the serum testosterone hormones were estimated, and the levels of oxidative stress biomarkers that take part in the reproductive pathologies such as catalase, glutathione, and malondialdehyde were estimated. Results The present results revealed that Al 2 O 3 -NPs induced DNA damage in testicular cells, marked histopathological alterations, and caused a significant elevation in MDA in testicular tissue. There was a significant decline in GSH and CAT activities. Furthermore, there was a significant decline in serum testosterone level in the testicular tissue of Al 2 O 3 -NP-administered rats. In contrast, pumpkin seed oil co-administration alleviated DNA damage and improved the histopathological alterations in the testicular tissues. Moreover, pumpkin seed oil co-administration significantly reduced MDA and improved the antioxidant defenses in testicular tissue. Conclusion The current study concluded that Al 2 O 3 -NPs caused testicular dysfunction by generating oxidative injury. Otherwise, PSO co-administration successfully attenuated the adverse impacts of Al 2 O 3 -NPs via suppression of oxidative stress and apoptosis as well as enhancement of the antioxidant defense system.

中文翻译：

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氧化铝纳米颗粒给药诱导白化大鼠睾丸功能障碍和南瓜籽油可能的保护作用

背景尽管纳米氧化铝在各个领域的广泛应用及其益处，但其对男性生殖系统的潜在影响信息有限。目的本研究旨在研究纳米氧化铝的睾丸功能障碍以及南瓜籽油（PSO）对氧化铝纳米颗粒（Al 2 O 3 -NPs）对雄性大鼠的潜在不利影响的保护作用。方法学 Al 2 O 3 -NPs 以 70 mg/kg 体重每天一次的剂量给大鼠口服给药，连续 28 天，而南瓜籽油在给药前以 4 mL/kg bw 的剂量给大鼠口服给药。 Al 2 O 3 -NPs，每天一次，连续 28 天。给药期后，精子浓度、活力、形态和 DNA 损伤，作为生殖毒性作用的生物标志物，使用精子分析和彗星试验进行评估，并对睾丸进行组织病理学检查。此外，还估计了血清睾酮激素的水平，并估计了参与生殖病理学的氧化应激生物标志物的水平，如过氧化氢酶、谷胱甘肽和丙二醛。结果目前的结果表明，Al 2 O 3 -NPs 诱导睾丸细胞中的DNA 损伤，显着的组织病理学改变，并导致睾丸组织中MDA 的显着升高。GSH和CAT活性显着下降。此外，Al 2 O 3 -NP给药大鼠的睾丸组织中的血清睾酮水平显着下降。相比之下，南瓜籽油共同给药减轻了 DNA 损伤并改善了睾丸组织的组织病理学改变。此外，南瓜籽油的共同给药显着降低了 MDA 并提高了睾丸组织的抗氧化防御能力。结论 目前的研究得出结论，Al 2 O 3 -NPs 通过产生氧化损伤导致睾丸功能障碍。否则，PSO 共同给药通过抑制氧化应激和细胞凋亡以及增强抗氧化防御系统成功地减弱了 Al 2 O 3 -NPs 的不利影响。