Although reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality. For the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased. The Beta diversity clusters of mice interfered by Folium sennae extracts were distinctly separated from control group. Combining PPI network analysis, cytochrome P450 enzymes metabolism was the main signaling pathway of diarrhea caused by Folium sennae. Moreover, 10 bacterial flora communities had statistical significant difference with Folium sennae intervention: the abundance of Paraprevotella, Streptococcus, Epulopiscium, Sutterella and Mycoplasma increased significantly; and the abundance of Adlercreutzia, Lactobacillus, Dehalobacterium, Dorea and Oscillospira reduced significantly. Seven of the 10 intestinal microbiota communities were related to the synthesis of tryptophan derivatives, which affected the transformation of aminotryptophan into L-tryptophan, leading to abnormal tryptophan metabolism in the host. Folium sennae targeted cytochrome P450 3A4 to alter intestinal bacterial characteristic and intervene the tryptophan metabolism of intestinal microbiota, such as Streptococcus, Sutterella and Dorea, which could be the intestinal microecological mechanism of diarrhea caused by Folium sennae extracts.

中文翻译：

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色氨酸代谢菌群在番泻叶提取物引起的小鼠腹泻中的作用。

尽管有报道提供了由番泻叶引起的腹泻可导致肠道菌群多样性紊乱的证据，但肠细菌的特性和具体机制仍不清楚。我们的研究目的是研究番泻叶引起的腹泻机制，这与肠道细菌特征重塑和代谢异常有关。对于番泻叶提取物的干预，Chao1指数和Shannon指数均下降。番泻叶提取物干扰的小鼠的Beta多样性簇与对照组明显分开。结合PPI网络分析，细胞色素P450酶代谢是番泻叶引起的腹泻的主要信号途径。此外，番泻叶干预对10个细菌菌群群落有统计学意义的显着变化：副球菌，链球菌，真核球菌，Sutterella和支原体的丰度显着增加。Adlercreutzia，Lactobacillus，Dehalobacterium，Dorea和Oscillospira的丰度明显降低。10个肠道微生物群落中的7个与色氨酸衍生物的合成有关，色氨酸衍生物的合成影响氨基色氨酸向L-色氨酸的转化，从而导致宿主中色氨酸代谢异常。番泻叶靶向细胞色素P450 3A4，以改变肠道细菌特性并干预肠道微生物群（例如链球菌，Sutterella和Dorea）的色氨酸代谢，