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In Vitro Biological Properties of Solanum sessiliflorum (Dunal), an Amazonian Fruit.
Journal of Medicinal Food ( IF 2.4 ) Pub Date : 2020-09-02 , DOI: 10.1089/jmf.2019.0193
Greice Franciele Feyh Dos Santos Montagner 1 , Fernanda Barbisan 2, 3 , Pauline Christ Ledur 3 , Aline Bolignon 4 , Jéssica de Rosso Motta 2 , Euler Esteves Ribeiro 5 , Raquel de Souza Praia 5 , Verônica Farina Azzolin 2 , Francine Carla Cadoná 6 , Alencar Kolinski Machado 6 , Rômulo Pillon Barcelos 7 , Ivana Beatrice Mânica da Cruz 2, 3
Affiliation  

Solanum sessiliflorum is an Amazonian fruit (cubiu) that has been domesticated since pre-Colombian era. It is also used in folk medicine to treat some clinical conditions. This investigation chemically characterized and analyzed the in vitro antioxidant and antitumoral effect of a cubiu pulp/seed hydroalcoholic extract. Cubiu extract was chemically characterized by high-performance liquid chromatography with diode array detector (HPLC-DAD), its antioxidant capacity measured by 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the following complementary in vitro protocols were performed: (1) cytoprotective effect of cubiu on human peripheral blood mononuclear cells (PBMCs) exposed to H2O2, a genotoxic and procarcinogen molecule; (2) effect of cubiu on low density lipoproteins oxidation; and (3) cytotoxic and antiproliferative effect on breast (MCF-7) and colorectal (HT-29) cancer cell lines. Biochemical and flow cytometry analyses were conducted in these protocols. Cubiu extract presented high concentrations of caffeic and gallic acids, beta-carotene, catechin, quercetin, and rutin, and its antioxidant capacity was confirmed. Cubiu attenuated H2O2 cytotoxicity on PBMCs, presented lowering effect on LDL oxidation, and induced mortality and proliferative inhibition of colorectal cancer cells. In cancer cells, cubiu extract at 10 μg/mL showed similar effects to 5-fluorouracil chemo drug reducing its viability and frequency of S-phase, indicating that cells are undergoing mitosis. In summary, despite the limitations of in vitro protocols, our results suggest that cubiu has several biological properties that affect human health.

中文翻译:

亚马孙水果Solanum sessiliflorum(Dunal)的体外生物学特性。

西洋是自前哥伦布时期以来就已驯化的亚马逊水果(cubiu)。它还在民间医学中用于治疗某些临床疾病。该研究从化学上表征并分析了cubiu纸浆/种子水醇提取物的体外抗氧化和抗肿瘤作用。使用高效液相色谱仪和二极管阵列检测器(HPLC-DAD)对Cuubiu提取物进行化学表征,通过2.2-二苯基-1-吡啶并肼基(DPPH)测定法测定其抗氧化能力,并进行以下补充性体外实验:立方对暴露于H 2 O 2的人外周血单个核细胞(PBMC)的细胞保护作用,具有遗传毒性和致癌物分子;(2)库比对低密度脂蛋白氧化的影响;(3)对乳腺癌(MCF-7)和结直肠癌(HT-29)癌细胞系的细胞毒性和抗增殖作用。在这些协议中进行了生化和流式细胞仪分析。库比提取物呈现出高浓度的咖啡酸和没食子酸,β-胡萝卜素,儿茶素,槲皮素和芦丁,并证实了其抗氧化能力。立方衰减的H 2 O 2对PBMC具有细胞毒性,降低LDL氧化作用,并诱导大肠癌细胞死亡和增殖抑制。在癌细胞中,浓度为10μg/ mL的cuubiu提取物显示出与5-氟尿嘧啶化学药物相似的作用,从而降低了其生存能力和S期频率,这表明细胞正在经历有丝分裂。总而言之,尽管体外方案存在局限性,我们的研究结果表明,cubiu具有几种影响人类健康的生物学特性。
更新日期:2020-09-10
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