DNA-PAINT’s imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.

中文翻译：

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在优化的DNA-PAINT中加速和多路复用高达100倍。

通过优化序列设计和缓冲条件，DNA-PAINT的成像速度最近得到了显着提高。但是，此实现方式尚未达到最终速度极限，仅适用于单个目标的成像。为了进一步提高采集速度，我们引入了串联的周期性DNA序列基序，与传统的DNA-PAINT相比，采样速度提高了100倍。我们将这种方法扩展到六个正交序列模体，现在可以实现速度优化的多路复用成像。