Precision-cut tissue slices are an important in vitro system to study organ function because they preserve most of the native cellular microenvironments of organs, including complex intercellular connections. However, during sample manipulation or slicing, some of the natural surface topology and structure of these tissues is lost or damaged. Here, we introduce a microfluidic platform to perform multiple assays on the surface of a tissue section, unhindered by surface topography. The device consists of a valve on one side and eight open microchannels located on the opposite side, with the tissue section sandwiched between these two structures. When the valve is actuated, eight independent microfluidic channels are formed over a tissue section. This strategy prevents cross-contamination when performing assays and enables parallelization. Using irregular tissues such as an aorta, we conducted multiple in vitro and ex vivo assays on tissue sections, including short-term culturing, a drug toxicity assay, a fluorescence immunohistochemistry staining assay, and an immune cell assay, in which we observed the interaction of neutrophils with lipopolysaccharide (LPS)-stimulated endothelium. Our microfluidic platform can be employed in other disciplines, such as tissue physiology and pathophysiology, morphogenesis, drug toxicity and efficiency, metabolism studies, and diagnostics, enabling the conduction of several assays with a single biopsy sample.

精确切割的组织切片是研究器官功能的重要体外系统，因为它们保留了器官的大部分天然细胞微环境，包括复杂的细胞间连接。然而，在样品操作或切片过程中，这些组织的一些自然表面拓扑结构和结构会丢失或损坏。在这里，我们引入了一个微流体平台，可以在组织切片的表面上进行多种检测，不受表面形貌的阻碍。该装置由一侧的阀门和位于另一侧的八个开放微通道组成，组织切片夹在这两个结构之间。当阀门启动时，在组织切片上形成八个独立的微流体通道。此策略可防止在执行检测时发生交叉污染并实现并行化。使用主动脉等不规则组织，我们对组织切片进行了多次体外和离体试验，包括短期培养、药物毒性试验、荧光免疫组织化学染色试验和免疫细胞试验，其中我们观察了相互作用中性粒细胞与脂多糖 (LPS) 刺激的内皮细胞。我们的微流体平台可用于其他学科，例如组织生理学和病理生理学、形态发生、药物毒性和效率、代谢研究和诊断，从而能够使用单个活检样本进行多项检测。在其中我们观察到中性粒细胞与脂多糖 (LPS) 刺激的内皮的相互作用。我们的微流体平台可用于其他学科，例如组织生理学和病理生理学、形态发生、药物毒性和效率、代谢研究和诊断，从而能够使用单个活检样本进行多项检测。在其中我们观察到中性粒细胞与脂多糖 (LPS) 刺激的内皮的相互作用。我们的微流体平台可用于其他学科，例如组织生理学和病理生理学、形态发生、药物毒性和效率、代谢研究和诊断，从而能够使用单个活检样本进行多项检测。