当前位置: X-MOL 学术Commun. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Non-invasive red-light optogenetic control of Drosophila cardiac function.
Communications Biology ( IF 5.9 ) Pub Date : 2020-06-29 , DOI: 10.1038/s42003-020-1065-3
Jing Men 1, 2 , Airong Li 3 , Jason Jerwick 2, 4 , Zilong Li 3 , Rudolph E Tanzi 3 , Chao Zhou 1, 2, 4
Affiliation  

Drosophila is a powerful genetic model system for cardiovascular studies. Recently, optogenetic pacing tools have been developed to control Drosophila heart rhythm noninvasively with blue light, which has a limited penetration depth. Here we developed both a red-light sensitive opsin expressing Drosophila system and an integrated red-light stimulation and optical coherence microscopy (OCM) imaging system. We demonstrated noninvasive control of Drosophila cardiac rhythms using a single light source, including simulated tachycardia in ReaChR-expressing flies and bradycardia and cardiac arrest in halorhodopsin (NpHR)-expressing flies at multiple developmental stages. By using red excitation light, we were able to pace flies at higher efficiency and with lower power than with equivalent blue light excitation systems. The recovery dynamics after red-light stimulation of NpHR flies were observed and quantified. The combination of red-light stimulation, OCM imaging, and transgenic Drosophila systems provides a promising and easily manipulated research platform for noninvasive cardiac optogenetic studies.



中文翻译:

果蝇心脏功能的非侵入性红光光遗传学控制。

果蝇是用于心血管研究的强大遗传模型系统。近来,已经开发了光遗传学起搏工具,以用有限的穿透深度的蓝光无创地控制果蝇的心律。在这里,我们开发了一种表达果蝇的对红光敏感的视蛋白以及一个集成的红光刺激和光学相干显微镜(OCM)成像系统。我们证明了果蝇的无创控制使用单个光源的心律,包括在表达ReaChR的果蝇中模拟的心动过速和在表达卤代视蛋白(NpHR)的果蝇中的心动过缓以及在多个发育阶段的心律骤停。与同等的蓝光激发系统相比,通过使用红激发光,我们能够以更高的效率和更低的功率对苍蝇进行起搏。观察并定量了NpHR果蝇的红光刺激后的恢复动力学。红光刺激,OCM成像和转基因果蝇系统的结合为无创性心脏光遗传学研究提供了一个有希望且易于操作的研究平台。

更新日期:2020-06-29
down
wechat
bug