Drosophila suzukii (spotted wing drosophila) has become a major invasive insect pest of soft fruits in the America and Europe, causing severe yield losses every year. The female D. suzukii shows the oviposition preference for ripening or ripe fruit by cutting the hard skin with its serrated ovipositor. A recent study reported that mechanosensation is involved in the texture discrimination during egg‐laying behaviour in D. suzukii. However, the underlying mechanism and molecular entity that control this behaviour are not known. The transient receptor potential (TRP) channels and degenerin/epithelial sodium channels (DEG/ENaC) are two candidate gene families of mechanically activated ion channels. Thus, we first identified TRP and DEG/ENaC genes in D. suzukii by bioinformatic analysis. Using transcriptome sequencing, we found that many TRP genes were expressed in the ovipositor in both D. suzukii and D. melanogaster, while some DEG/ENaCs showed species‐specific expression patterns. Exposure to drugs targeting TRP and DEG/ENaC channels abolished the oviposition preference for harder texture in female D. suzukii. Therefore, mechanosensitive ion channels may play significant roles in the texture assessment of egg‐laying behaviour in D. suzukii, which has promising implications to further research on the development of novel control measures.

中文翻译：

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确定潜在的机械敏感离子通道参与果蝇铃木产卵行为期间的纹理识别。

铃木果蝇（斑翅果蝇）已成为美洲和欧洲软果的主要入侵害虫，每年造成严重的产量损失。雌性铃木D.通过锯齿状产卵器切开硬皮，表现出对成熟或成熟水果的产卵偏爱。最近的一项研究报道，在铃木D.产卵过程中，机械传感与纹理识别有关。但是，尚不清楚控制此行为的潜在机理和分子实体。瞬时受体电位（TRP）通道和简并/上皮钠通道（DEG / ENaC）是机械激活离子通道的两个候选基因家族。因此，我们首先确定了铃木D.TRP和DEG / ENaC基因通过生物信息学分析。通过转录组测序，我们发现许多DRP基因在铃木和黑腹果蝇的产卵器中都有表达，而一些DEG / ENaCs表现出物种特异性的表达模式。接触靶向TRP和DEG / ENaC通道的药物消除了雌性铃木D.偏爱较硬质地的产卵倾向。因此，机械敏感离子通道可能在铃木D.产卵行为的质构评估中起重要作用，这对进一步研究开发新型控制措施具有潜在的意义。