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A volatolomic approach using cerumen as biofluid to diagnose bovine intoxication by Stryphnodendron rotundifolium.
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-06-29 , DOI: 10.1002/bmc.4935 João Marcos G Barbosa 1 , Milenna K Fernandes Rodrigues 2 , Lurian C David 1 , Taynara C E Silva 1 , Danielly A Fortuna Lima 1 , Naiara Z Pereira 1 , Emmanuel B D'Alessandro 1 , Anselmo E de Oliveira 3 , Paulo H Jorge da Cunha 2 , Maria Clorinda S Fioravanti 2 , Nelson R Antoniosi Filho 1
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-06-29 , DOI: 10.1002/bmc.4935 João Marcos G Barbosa 1 , Milenna K Fernandes Rodrigues 2 , Lurian C David 1 , Taynara C E Silva 1 , Danielly A Fortuna Lima 1 , Naiara Z Pereira 1 , Emmanuel B D'Alessandro 1 , Anselmo E de Oliveira 3 , Paulo H Jorge da Cunha 2 , Maria Clorinda S Fioravanti 2 , Nelson R Antoniosi Filho 1
Affiliation
An innovative volatolomic approach employs the detection of biomarkers present in cerumen (earwax) to identify cattle intoxication by Stryphnodendron rotundifolium Mart., Fabaceae (popularly known as barbatimão). S. rotundifolium is a poisonous plant with the toxic compound undefined and widely distributed throughout the Brazilian territory. Cerumen samples from cattle of two local Brazilian breeds (‘Curraleiro Pé‐Duro’ and ‘Pantaneiro’) were collected during an experimental intoxication protocol and analyzed using headspace (HS)/GC–MS followed by multivariate analysis (genetic algorithm for a partial least squares, cluster analysis, and classification and regression trees). A total of 106 volatile organic metabolites were identified in the cerumen samples of bovines. The intoxication by S. rotundifolium influenced the cerumen volatolomic profile of the bovines throughout the intoxication protocol. In this way, it was possible to detect biomarkers for cattle intoxication. Among the biomarkers, 2‐octyldecanol and 9‐tetradecen‐1‐ol were able to discriminate all samples between intoxicated and nonintoxicated bovines. The cattle intoxication diagnosis by S. rotundifolium was accomplished by applying the cerumen analysis using HS/GC–MS, in an easy, accurate, and noninvasive way. Thus, the proposed bioanalytical chromatography protocol is a useful tool in veterinary applications to determine this kind of intoxication.
中文翻译:
一种挥发性的方法,使用铈作为生物流体来诊断圆角头孢菌素对牛的中毒。
一种创新的挥发性方法,通过检测存在于陶瓷(耳垢)中的生物标志物来鉴定Fabaceae的圆头花轮虫市场(通常称为barbatimão)对牛的中毒作用。圆形沙门氏菌是一种有毒植物,其毒性化合物不确定,并广泛分布于整个巴西领土。在实验性中毒方案中收集了两个巴西地方品种(“ CurraleiroPé-Duro”和“ Pantaneiro”)牛的血清样品,并使用顶空(HS)/ GC-MS分析,然后进行多变量分析(遗传算法用于部分最小平方,聚类分析以及分类和回归树)。在牛的陶瓷样品中总共鉴定出106种挥发性有机代谢产物。圆形葡萄球菌中毒在整个中毒协议中影响了牛的um挥发物谱。这样,可以检测牛中毒的生物标志物。在生物标志物中,2-辛基癸醇和9-十四烯-1-醇能够区分所有样品在牛和非牛中。通过使用HS / GC-MS进行血清分析,以一种简便,准确且无创的方式完成了圆形葡萄球菌对牛的中毒诊断。因此,所提出的生物分析色谱方案是兽医应用中确定这种中毒的有用工具。
更新日期:2020-06-29
中文翻译:
一种挥发性的方法,使用铈作为生物流体来诊断圆角头孢菌素对牛的中毒。
一种创新的挥发性方法,通过检测存在于陶瓷(耳垢)中的生物标志物来鉴定Fabaceae的圆头花轮虫市场(通常称为barbatimão)对牛的中毒作用。圆形沙门氏菌是一种有毒植物,其毒性化合物不确定,并广泛分布于整个巴西领土。在实验性中毒方案中收集了两个巴西地方品种(“ CurraleiroPé-Duro”和“ Pantaneiro”)牛的血清样品,并使用顶空(HS)/ GC-MS分析,然后进行多变量分析(遗传算法用于部分最小平方,聚类分析以及分类和回归树)。在牛的陶瓷样品中总共鉴定出106种挥发性有机代谢产物。圆形葡萄球菌中毒在整个中毒协议中影响了牛的um挥发物谱。这样,可以检测牛中毒的生物标志物。在生物标志物中,2-辛基癸醇和9-十四烯-1-醇能够区分所有样品在牛和非牛中。通过使用HS / GC-MS进行血清分析,以一种简便,准确且无创的方式完成了圆形葡萄球菌对牛的中毒诊断。因此,所提出的生物分析色谱方案是兽医应用中确定这种中毒的有用工具。