A novel bacteriophage vB_VpaS_PG07 (hereafter designated PG07) that infects Vibrio parahaemolyticus was isolated. The bacteriophage was examined by transmission electron microscopy, and the result showed that PG07 belonged to family Siphoviridae, with an isometric polyhedral head (80 nm in diameter) and a long tail (175 nm in length). The one-step growth curve showed that the latent period and burst size were 10 min and 60 PFUs/infected cell, respectively. PG07 had double-stranded DNA genome of 112, 106 bp with 43.65 % G+C content. A total of 158 putative open reading frames (ORFs) were identified in the genome of PG07, including functional genes associated with integration, nucleotide metabolism and replication, structure and packaging and bacterial lysis. Sixteen tRNA genes were discovered, and no genes associated with pathogenicity and virulence were identified. The genome of PG07 showed very low similarity to phage genomes deposited in public databases (77.65 % nucleotide identity and 9 % query coverage). The newly sequenced PG07 could be considered as a novel T5-like virus. PG07 significantly reduced the mortality of shrimps challenged with V. parahaemolyticus, a bacterium causing acute hepatopancreatic necrosis disease (AHPND). The findings highlight the potential of PG07 as an effective antibacterial agent for phage prophylaxis and phage therapy in aquaculture.

分离了感染副溶血性弧菌的新型噬菌体vB_VpaS_PG07（以下称为PG07）。用透射电镜检查了噬菌体，结果表明PG07属于Siphoviridae科。，具有等距的多面体头（直径80 nm）和长尾巴（长度175 nm）。一步生长曲线表明潜伏期和爆发大小分别为10分钟和60 PFU /感染细胞。PG07具有112、106 bp的双链DNA基因组，G + C含量为43.65％。在PG07的基因组中总共鉴定了158个推定的开放阅读框（ORF），包括与整合，核苷酸代谢和复制，结构和包装以及细菌裂解有关的功能基因。发现了16个tRNA基因，没有发现与致病性和毒力相关的基因。PG07的基因组与公共数据库中保存的噬菌体基因组的相似性非常低（77.65％的核苷酸同一性和9％的查询覆盖率）。新测序的PG07可以被认为是新型的T5样病毒。V. parahaemolyticus，引起急性肝胰腺坏死病（AHPND）的细菌。这些发现凸显了PG07作为水产养殖中预防和预防噬菌体的有效抗菌剂的潜力。