2-(4-((4-Chloro-6-((3',6'-dihydroxy-3-oxospiro [isoindoline-1,9'-xanthen]-2-yl)amino)-1,3,5-triazin-2-yl)amino)benzoyl)-N-phenylhydrazine-1-carbothioamide (PCFH) is prepared for the detection of Hg²⁺ by hydrogen-induced emission (HIE). In the presence of blood serum, PCFH has a strong fluorescence response to Hg²⁺ in ethanol/H₂O (90/10, v/v), while the fluorescence with other metal ions, including Cu²⁺, is much weaker. PCFH coordinates better with Hg²⁺ than Cu²⁺, which gives rise to specificity of detection. Through spectroscopic techniques and molecular dynamics simulations, the mechanism of HIE reveals that the interactions between polar bonds of PCFH and protein in blood serum via hydrogen bonding can efficiently enhance the specificity and sensitivity of detection. As the results of the interactions, the free rotation of two plenary structures of PCFH linked by CC bonds is restricted, which leads to increased planarity, and the protein destroys the coordination between Cu²⁺ and PCFH. The development of cells under Hg²⁺ pollution is monitored, and the concentrations of Hg²⁺ in deformed and dead cells are 0.47 μM and 0.88 μM respectively. The HIE effects found here will promote new ideas in the design of new types of fluorescence probes for Hg²⁺ and other metal ions.

2-（4-（（4-氯-6-（（3'，6'-二羟基-3-氧代螺[异吲哚啉-1,9'-黄嘌呤] -2-基）氨基）-1,3,5-制备了三嗪-2-基）氨基）苯甲酰基）-N-苯基肼-1-碳硫酰胺（PCFH），用于通过氢诱导发射（HIE）检测Hg ²⁺。在存在血清的情况下，PCFH在乙醇/ H ₂ O（90/10，v / v）中对Hg ²⁺具有很强的荧光响应，而与其他金属离子（包括Cu ²⁺）的荧光要弱得多。PCFH与Hg ^2+的配合比与Cu ^2+的配合更好，这引起了检测的特异性。通过光谱技术和分子动力学模拟，HIE的机理揭示了PCFH极性键与血清中蛋白质之间的氢键相互作用可以有效提高检测的特异性和灵敏度。作为相互作用的结果，通过C C键连接的PCFH的两个完整结构的自由旋转受到限制，这导致平面度增加，并且蛋白质破坏了Cu ²⁺与PCFH之间的配位。监测Hg ²⁺污染下细胞的发育，并检测Hg ²⁺的浓度变形和死细胞中的α分别为0.47μM和0.88μM。此处发现的HIE效应将为设计用于Hg ²⁺和其他金属离子的新型荧光探针带来新的思路。