The WRKY transcription factors (TFs) are involved in aluminum (Al) stress and jasmonic acid (JA)-regulated resistance responses. WRKYs act as regulators of Al-activated malate transporter (ALMT) proteins (anion channels) by directly binding to their promoters and altering malate efflux, thereby regulating Al ion toxicity in plant roots. JA enhances Al-induced root growth inhibition in Arabidopsis. However, the relationship between WRKY and ALMT genes and their involvement in JA-mediated root growth inhibition during Al stress in tomato remain unknown. Here, we demonstrate a similar phenomenon that JA enhances Al-induced root growth inhibition in tomato (Solanum lycopersicum). By analyzing RNA-seq data and tissue-specific expression data from public databases, we selected 17 WRKY and 6 ALMT family genes to identify the genes participated in this process. The promoters of many of the selected genes contained MeJA responsive element, G-box (target site of MYC2, a core TF of JA signaling), and W-box (target site for WRKY). Quantitative real-time PCR was performed to evaluate the expression levels of selected WRKY and ALMT genes under AlCl₃ and Methyl jasmonate (MeJA) treatment. SlMYC2-VIGS seedlings and jasmonic acid-insensitive1 (jai1) mutant were also employed to analyze the expression patterns of selected genes. We find that SlALMT3 is responsible for the crosstalk regulatory mechanism between Al and JA in root growth inhibition, and 6 SlWRKYs may act as the upstream regulators of SlALMT3 in this crosstalk response. This study is initial and informative in exploring the crosstalk regulatory mechanism between JA and Al in tomato.

WRKY转录因子（TFs）参与铝（Al）胁迫和茉莉酸（JA）调节的抗性反应。WRKY通过直接与其启动子结合并改变苹果酸外排，从而充当Al激活的苹果酸转运蛋白（ALMT）蛋白（阴离子通道）的调节剂，从而调节植物根部的Al离子毒性。JA增强拟南芥中铝诱导的根生长抑制。然而，WRKY和ALMT基因之间的关系以及它们在番茄铝胁迫过程中参与JA介导的根生长抑制的关系仍然未知。在这里，我们证明了一个类似的现象，即JA增强了番茄对铝诱导的根系生长的抑制作用（茄属植物lycopersicum）。通过分析来自公共数据库的RNA-seq数据和组织特异性表达数据，我们选择了17个WRKY和6个ALMT家族基因来鉴定参与该过程的基因。许多选定基因的启动子包含MeJA响应元件，G-box（MYC2的靶位点，JA信号转导的核心TF）和W-box（WRKY的靶位）。在AlCl ₃和茉莉酸甲酯（MeJA）处理下，进行实时定量PCR评估所选WRKY和ALMT基因的表达水平。还使用了SlMYC2-VIGS幼苗和茉莉酸不敏感1（jai1）突变体来分析所选基因的表达模式。我们发现SlALMT3负责根的生长抑制Al和JA之间的串扰的调节机制，和6个SlWRKYs可以为一体的上游调节作用SlALMT3在此串扰响应。这项研究是探索番茄中JA与Al之间的串扰调节机制的初步和有益的信息。