Abstract Rhodamine B hydrazide (RBH) was prepared as a new derivatizing reagent for residual formaldehyde in a drug such as N-butyl-2-cyanoacrylate (NBCA). RBH reacted with formaldehyde rapidly at room temperature, and the resulting derivative can be detected sensitively at 238 nm. After optimizing the derivatization and chromatographic separation conditions, RBH as a pre-column derivatizing reagent has been successfully used for the analysis of formaldehyde in real NBCA samples by high-performance liquid chromatography (HPLC). The detection limit of the method is 0.004 μg/mL for formaldehyde, and the recovery is 94.0%-96.6%. Most notably, compared with the previous HPLC methods, the developed method has the advantages of mild derivatization temperature, short derivatization time and high sensitivity.

中文翻译：

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罗丹明B酰肼高效液相色谱法检测2-氰基丙烯酸正丁酯中的残留甲醛

摘要 制备了罗丹明B酰肼(RBH)作为药物如N-丁基-2-氰基丙烯酸酯(NBCA)中残留甲醛的新型衍生试剂。RBH 在室温下与甲醛快速反应，生成的衍生物可在 238 nm 处灵敏检测。在优化衍生化和色谱分离条件后，RBH作为柱前衍生化试剂已成功用于高效液相色谱法（HPLC）分析真实NBCA样品中的甲醛。该方法对甲醛的检出限为0.004 μg/mL，回收率为94.0%-96.6%。最值得注意的是，与以往的HPLC方法相比，该方法具有衍生温度温和、衍生时间短、灵敏度高等优点。