Abstract Peste des petits ruminants (PPR) virus has a surface glycoprotein, hemagglutinin neuraminidase (HN) with functional sites for cell attachment and neuraminidase (NA) activity. The neuraminidase activity of PPR virus (Sungri/96) was biochemically characterized with fluorometric assay, enzyme linked lectin assay (ELLA) and electrochemical impedance spectroscopy (EIS). Furthermore, the inhibitory effect of gallic acid on the virus neuraminidase activity was also evaluated in-vitro in B95a cells. Screen printed carbon electrode were used for the EIS assay. The activity of the viral neuraminidase was presented as the change in charge transfer resistance (Rct). The measurement of neuraminidase activity with EIS was found comparable to ELLA. Cell toxicity (CC50) concentration of gallic acid was found to be 61.0 ± 1.0 µM in B95a cells. A dose dependent effect of gallic acid on the virus neuraminidase activity was observed in both EIS and ELLA measurement. The anti PPRV activity of gallic acid was quantified by cell ELISA and 7.0 ± 1.0 µM was calculated concentration that has 50% viral inhibition in B95a cells. The gallic acid used in present study had neuraminidase inhibitory activity both in ELLA and EIS, furthermore it also reduces the virus propagation in vitro in B95a cells. For high throughput screening of anti-neuraminidase compounds including phytocompounds, electrochemical impedance spectroscopy could be vividly used.

中文翻译：

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用于监测 PPR HN 蛋白的没食子酸抑制神经氨酸酶活性的电化学阻抗传感器

摘要 小反刍兽疫 (PPR) 病毒具有表面糖蛋白、血凝素神经氨酸酶 (HN)，具有用于细胞附着和神经氨酸酶 (NA) 活性的功能位点。PPR 病毒 (Sungri/96) 的神经氨酸酶活性通过荧光测定、酶联凝集素测定 (ELLA) 和电化学阻抗谱 (EIS) 进行生化表征。此外，还在 B95a 细胞中体外评估了没食子酸对病毒神经氨酸酶活性的抑制作用。丝网印刷碳电极用于EIS测定。病毒神经氨酸酶的活性表现为电荷转移电阻 (Rct) 的变化。发现使用 EIS 测量神经氨酸酶活性与 ELLA 相当。发现 B95a 细胞中没食子酸的细胞毒性 (CC50) 浓度为 61.0 ± 1.0 µM。在 EIS 和 ELLA 测量中均观察到没食子酸对病毒神经氨酸酶活性的剂量依赖性影响。没食子酸的抗 PPRV 活性通过细胞 ELISA 进行量化，7.0 ± 1.0 µM 被计算为在 B95a 细胞中具有 50% 病毒抑制的浓度。本研究中使用的没食子酸在 ELLA 和 EIS 中均具有神经氨酸酶抑制活性，此外它还减少了 B95a 细胞中病毒的体外传播。对于包括植物化合物在内的抗神经氨酸酶化合物的高通量筛选，可以生动地使用电化学阻抗谱。0 µM 是在 B95a 细胞中具有 50% 病毒抑制的计算浓度。本研究中使用的没食子酸在 ELLA 和 EIS 中均具有神经氨酸酶抑制活性，此外它还减少了 B95a 细胞中病毒的体外传播。对于包括植物化合物在内的抗神经氨酸酶化合物的高通量筛选，可以生动地使用电化学阻抗谱。0 µM 是在 B95a 细胞中具有 50% 病毒抑制的计算浓度。本研究中使用的没食子酸在 ELLA 和 EIS 中均具有神经氨酸酶抑制活性，此外它还减少了 B95a 细胞中病毒的体外传播。对于包括植物化合物在内的抗神经氨酸酶化合物的高通量筛选，可以生动地使用电化学阻抗谱。