Tracking the in vivo transportation and localization of microplastics is the prerequisite for a better understanding of the toxicity but has not been reported yet. Fluorescent microbeads are widely used for tracking in the laboratory, whereas the most abundant microplastic (e.g., fiber) found in the field is rarely adapted. In this study, we used fiber slicer and blades to cut spinning materials into fluorescent micro-sized fibers with various lengths. Fiber slicer and blades are efficient tools to produce specific lengths of fibers. The microfibers showed a specific color under the ultraviolet irradiation; therefore, these microfibers can be clearly visualized when they are within aquatic organisms, such as water flea, shrimp, medaka, and zebrafish, which enables us to observe the whole biological processes of microfibers directly. Thus, the present study provides a novel method of microfibers preparation, and this kind of fluorescent fiber is applicable to be an efficient and convenient tracer for in vivo process observation and potential toxicological analysis in the future.

体内追踪微塑料的运输和定位是更好地了解毒性的前提，但尚未见报道。荧光微珠在实验室中被广泛用于跟踪，而在现场发现的最丰富的微塑料（例如纤维）很少被采用。在这项研究中，我们使用纤维切片机和刀片将纺丝材料切割成各种长度的荧光微型纤维。纤维切片机和刀片是生产特定长度纤维的有效工具。在紫外线照射下，微纤维显示出特定的颜色。因此，当它们位于水生生物（如水蚤，虾，shrimp，斑马鱼）中时，这些微纤维可以清晰地看到，这使我们能够直接观察微纤维的整个生物过程。从而，未来的体内过程观察和潜在的毒理学分析。