The ability to utilize agricultural waste products is one of the many reasons why bioprocessing using Escherichia coli is an attractive alternative to unsustainable fossil fuel-based methods for chemical synthesis. However, mixed sugar co-utilization is a challenge for E. coli due to the effects of carbon catabolite repression (CCR), which dictates a preference for the consumption of glucose over secondary sugars through the mechanisms of inducer exclusion and transcriptional regulation. To overcome these obstacles, metabolic engineers have developed strategies to relax the effects of CCR through both global and sugar-specific methods. Understanding the feed conditions as well as the engineering opportunities for CCR relaxation can allow for the development of a strain with maximized productivity and growth.

利用农业废弃物的能力是为什么使用大肠杆菌进行生物处理是基于不可持续的基于化石燃料的化学合成方法的有吸引力的替代方法的众多原因之一。但是，混合糖的联合利用对大肠杆菌是一个挑战由于碳分解代谢物阻遏（CCR）的作用，它通过诱导剂排斥和转录调控机制决定了葡萄糖的消耗优先于次级糖。为了克服这些障碍，新陈代谢工程师已经开发出通过整体方法和特定于糖的方法来放松CCR效应的策略。了解进料条件以及CCR松弛的工程机会可以使菌株的生产具有最大的生产率和生长率。