Biofuel production particularly biodiesel has been a subject of investigation worldwide in order to envisage an ecofriendly, renewable source of fuel. Microalgae are one of the key organisms to be utilized in biodiesel production. In the present study, Tetraselmis sp., marine microalgae was investigated for its biodiesel production using the two-stage process optimization method. The first stage consisted of nutrient supplementation with optimization of media (dilution of F/2 media with simulated dairy wastewater) while in second stage, stress factors (nutrient starvation, salinity, pH) were employed with the optimization of factors using response surface methodology. Tetraselmis sp. was grown on different percentage of dairy wastewater and 75% dilution served as better media for growth. The experimental results highlighted that the interaction of nutrient starvation along with salinity was important (p < 0.05). Lipid content was 51.65% in validation experiment and the COD removal was 95% and the major fatty acids were C-16:0, C-18:0, C-18:1.Tetraselmis sp. can be a promising organism for the production of biodiesel coupled with bioremediation of dairy wastewater.

为了设想一种生态友好的可再生燃料来源，生物燃料生产，特别是生物柴油已成为全世界研究的主题。微藻是生物柴油生产中使用的关键生物之一。在本研究中，使用两阶段工艺优化方法研究了海洋微藻Tetraselmis sp。的生物柴油生产。第一阶段包括营养的补充和培养基的优化（用模拟的乳品废水稀释F / 2培养基），而第二阶段则采用应力因子（营养饥饿，盐度，pH），并使用响应面法对因子进行优化。四翅目sp。在不同百分比的乳品废水中生长，75％的稀释液是生长的更好培养基。实验结果表明，营养饥饿与盐分的相互作用非常重要（p <0.05）。验证实验中脂质含量为51.65％，COD去除率为95％，主要脂肪酸为C-16：0，C-18：0，C-18：1。Tetraselmis sp。可以成为生产生物柴油以及对牛奶废水进行生物修复的有前途的生物。