Aspergillus carbonarius is an efficient producer of organic acids with great potential for bio-based production of organic acids. In this study, we identified a gene f2kp encoding the enzyme 6-phosphofructo-2-kinase known as an allosteric regulator of the glycolytic flux and investigated its role in the production of organic acid. The strategy was to examine the impact of citric acid and malic acid production by overexpressing and disrupting f2kp, respectively. The overexpressing transformants expressed f2kp at higher level than the wild type, whereas no expression of f2kp was detected in the knockout transformants. Citric acid and malic acid production by the knockout strains decreased sharply along with a significant lower sugar consumption, though the overexpressing transformants produced similar amounts of citric acid and malic acid as the wild type. We conclude that 6-phosphofructo-2-kinase has an important regulatory role for the glycolytic flux and organic acid production in A. carbonarius.

中文翻译：

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6-磷酸果糖-2-激酶的破坏和过表达影响炭黑曲霉 ITEM 5010 中有机酸的产生

Aspergillus carbonarius 是一种高效的有机酸生产者，在生物基有机酸生产方面具有巨大潜力。在这项研究中，我们确定了一个基因 f2kp，该基因编码酶 6-phosphofructo-2-kinase，该酶被称为糖酵解通量的变构调节剂，并研究了其在有机酸生产中的作用。该策略是通过分别过表达和破坏 f2kp 来检查柠檬酸和苹果酸生产的影响。过表达的转化体以高于野生型的水平表达 f2kp，而在敲除转化体中未检测到 f2kp 的表达。敲除菌株的柠檬酸和苹果酸产量急剧下降，糖消耗量显着降低，尽管过表达的转化体产生的柠檬酸和苹果酸量与野生型相似。我们得出结论，6-phosphofructo-2-kinase 对 A. carbonarius 中的糖酵解通量和有机酸产生具有重要的调节作用。