We investigated the effect of inoculation with the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245 under aeroponic conditions on the quality and performance of minitubers of potato (Solanum tuberosum L. cvs. Nevsky and Kondor). In vitro-grown microplants were planted in an aeroponic system, and after 3 weeks of growth, the bacteria were added to the potato nutrient solution. We evaluated the effect of inoculation on the potato growth variables, minituber yield and minituber starch content. Root-associated bacteria were detected by confocal microscopy, and their numbers in the plant growth medium were monitored by enzyme-linked immunosorbent assay. Cells of A. brasilense Sp245 remained on roots during plant growth and were eliminated from the nutrient solution in the aeroponic system within a week after inoculation. Inoculation brought about statistically significant increases in shoot height (16%) and in leaf blade number (14%). The number of minitubers increased by 40% in Kondor plants and by 60% in Nevsky plants, and their yield per square metre increased by an average of 17% in both cultivars. Inoculation also increased the size of starch grains in the amyloplastids (by 12%) and the starch content of minitubers (by 1.5 times). The plant-bacterial association developed in this study was active under aeroponic conditions. This report is the first to show that A. brasilense Sp245 promotes the growth, development and performance of potato and helps to increase the quality of minitubers, thereby contributing to the efficacy of potato seed tuber production.

我们研究了在有氧条件下接种促进植物生长的根瘤菌巴西细螺旋藻Sp245对马铃薯微型块茎（Solanum tuberosum L. cvs。Nevsky and Kondor）的品质和性能的影响。将体外生长的微型植物种植在航空系统中，生长3周后，将细菌添加到马铃薯营养液中。我们评估了接种对马铃薯生长变量，小块茎产量和小块茎淀粉含量的影响。通过共聚焦显微镜检测根相关细菌，并通过酶联免疫吸附测定法监测其在植物生长培养基中的数量。巴西拟南芥的细胞Sp245在植物生长过程中保留在根部，并在接种后一周内从航空系统的营养液中清除。接种引起枝高（16％）和叶片数量（14％）的统计学显着增加。康多植物中的微型块茎数量增加了30％，涅夫斯基植物中的微型块茎数量增加了60％，两个品种的平均每平方米产量平均增加了17％。接种还增加了淀粉状体中淀粉粒的大小（增加了12％）和微型块茎的淀粉含量（增加了1.5倍）。在这项研究中建立的植物细菌协会在有氧环境下是活跃的。该报告是第一个显示巴西青霉菌的报告 Sp245促进马铃薯的生长，发育和性能，并有助于提高微型块茎的品质，从而有助于马铃薯种子块茎生产的功效。