Mambalgin-1 is a peptide that acts as a potent analgesic through inhibiting acid-sensing ion channels (ASIC) in nerve cells. Research has shown that ASIC channels are involved in the proliferation and growth of cancer cells; therefore, Mambalgin-1 can be a potential anti-cancer by inhibiting these channels. In the present study, the Nicotiana benthamiana codon optimized Mambalgin-1 gene was synthesized and cloned in PVX (potato virus X) viral vector. The two cultures of Agrobacterium containing Mambalgin-1 and P19 silencing suppressor genes were co-agroinfiltrated into N. benthamiana leaves. Five days post infiltration, the production of recombinant Mambalgin-1 was determined by western blotting. For biological activity, MTT (3(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide) was analyzed for the cytotoxicity recombinant Mambalgin-1 from the transformed plants on nervous (SH-SY5Y) and breast (MCF7) cancer cells. The results showed that the plants expressing open reading frame of Mambalgin-1 showed recombinant 7.4 kDa proteins in the entire plant extract. In the MTT test, it was found that Mambalgin-1 had cytotoxic effects on SH-SY5Y cancer cells, yet no effects on MCF7 cancer cells were observed. According to the results, the expression of the biologically active recombinant Mambalgin-1 in the transformed plant leaves was confirmed and Mambalgin-1 can also have anti-cancer (inhibition of ASIC channels) potential along with its already known analgesic effect.

Mambalgin-1 是一种肽，通过抑制神经细胞中的酸敏感离子通道 (ASIC) 发挥有效镇痛作用。研究表明，ASIC 通道参与癌细胞的增殖和生长；因此，Mambalgin-1 可以通过抑制这些通道成为一种潜在的抗癌药物。在本研究中，合成了本氏烟草密码子优化的Mambalgin-1基因，并将其克隆到PVX（马铃薯病毒X）病毒载体中。将含有 Mambalgin-1 和 P19 沉默抑制基因的两种农杆菌培养物共同农杆菌渗入本氏烟草树叶。浸润后五天，通过蛋白质印迹测定重组Mambalgin-1的产生。对于生物活性，分析 MTT (3(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide) 对神经 (SH-SY5Y) 和乳腺 (MCF7) 转化植物的细胞毒性重组 Mambalgin-1 ) 癌细胞。结果表明，表达Mambalgin-1开放阅读框的植物在整个植物提取物中显示出重组的7.4 kDa蛋白。MTT试验发现Mambalgin-1对SH-SY5Y癌细胞有细胞毒作用，但对MCF7癌细胞没有作用。根据结果​​，