Living tissues in the body receive various types of stimuli, including mechanical strain, pressure, and varied chemical environments. In contrast, conventional cell cultures are processed under in vitro conditions, which are not similar to the actual body’s environment. To precisely simulate the human body environment, a dynamic cell culture device capable of applying mechanical stimulation to cells is needed. In this study, an acrylic dielectric elastomer electroactive polymer (EAP), is introduced as a driving component for a dynamic cell culture device with a simple structure. The device is composed of separated upper and lower modules with a driving film at the center, By assembling these components, the electrodes on the surface of the driving film are isolated but still connected by columns that can transfer the deformation of the driving apparatus to the culture membrane. The culturing performance of cells according to the mechanical stimuli was experimentally investigated and compared. Tensile strain was found to provide the highest improvement in cell development rate, reaching up to 32.3%. These results highlight the utility of EAPs for compact and biocompatible dynamic cell culture device design.

体内的活组织受到各种类型的刺激，包括机械应变，压力和变化的化学环境。相比之下，常规细胞培养是在体外条件下进行的，这与实际人体环境不同。为了精确地模拟人体环境，需要一种能够对细胞施加机械刺激的动态细胞培养装置。在这项研究中，丙烯酸介电弹性体电活性聚合物（EAP）被引入作为具有简单结构的动态细胞培养设备的驱动组件。该装置由分离的上下模块组成，中间有驱动膜，通过组装这些组件，驱动膜表面上的电极是隔离的，但仍通过可将驱动装置的变形传递到培养膜的柱连接。实验研究并比较了根据机械刺激对细胞的培养性能。发现拉伸菌株可提供最高的细胞发育速率改善，最高可达32.3％。这些结果突出了EAP在紧凑型和生物相容性动态细胞培养设备设计中的实用性。