Buxus spp. are possibly endangered by fungal pathogens of the genus Calonectria. The first documented occurrence of the pathogen Calonectria pseudonaviculata in the Czech Republic, in 2010, was on young plants in a plant production nursery. In 2017 and 2018, Calonectria spp. were monitored on established landscape plants in 27 historical gardens. Leaf spots and dieback of the plants were recorded in 20 historical gardens. Parts of symptomatic boxwood plants were collected and pathogens present isolated. Four fungal isolates were morphologically identified as Calonectria sp. and distinguished as Calonectria pseudonaviculata and C. henricotiae by enzyme restriction within the β–tubulin PCR amplicons, sequencing, and phylogenetic analysis. The results showed that at present, both species of Calonectria pathogenic to boxwood occur in the Czech Republic.

黄杨属 可能被Calonectria属的真菌病原体危害。2010年，捷克共和国首次有记载的病原体假单胞菌Calonectria pseudonaviculata发生在植物生产苗圃的年轻植物上。在2017年和2018年，Calonectria spp。对27个历史花园中已建立的园林植物进行了监测。在20个历史花园中记录了植物的叶斑和枯萎。收集部分有症状的黄杨木植物，并分离出目前的病原体。在形态上鉴定出四个真菌分离物为Calonectria sp。并被区分为Calonectria pseudonaviculata和C. henricotiae通过β-微管蛋白PCR扩增子中的酶限制，测序和系统发育分析。结果表明，目前，对黄杨木有致病作用的Calonectria两种物种都在捷克共和国发生。