当前位置: X-MOL 学术Euphytica › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
QTL analysis of rice photosynthesis-related traits under the cold stress across multi-environments
Euphytica ( IF 1.9 ) Pub Date : 2020-06-29 , DOI: 10.1007/s10681-020-02651-5
Zhaoguo Wang , Shuo Wang , Chunhong Yu , Xiao Han , Detang Zou

Cold stress is one of the major abiotic stress factors influencing photosynthesis in rice. In the present study, a linkage analysis and genome-wide association study (GWAS) of photosynthesis-related traits (PRT) at the grain filling stage of rice, including net photosynthetic rate (Pn), stomatal conductance (Sc), transpiration rate (Tr), and intercellular CO 2 concentration (Ci), were performed under cold stress using a recombinant inbred line population and single-nucleotide polymorphism (SNP) re-sequencing data of 295 japonica varieties. Twenty-nine additive QTLs (A-QTLs) were detected, and 17 out of the 29 QTLs were cold stress-related. qPn7 - 1 , qTr1 , qTr7 - 1 , qTr7 - 2 , qCi7 - 1 and qSc7 - 1 were specifically expressed under the cold stress and control conditions in different years, with one major pleiotropic QTL interval (RM1306–RM70) detected. In addition, additive × environment interaction QTLs revealed that qPn7 - 1 , qTr1 , qTr7 - 1 , qCi7 - 1 , qSc7 - 1 , and qSc8 - 1 were influenced mainly by genetic factors, suggesting they could be exploited in cold tolerance breeding activities. Ten SNPs were associated significantly with PRT under cold-water stress based on GWAS, and three out the ten were included in the major QTL interval of RM1306–RM70, verifying the QTLs detected in multiple environments by linkage analysis. According to the major QTL genotypes linked to PRT, phenotypically superior RIL47 could be considered for genetic improvement of PRT under cold water stress. The results could facilitate the fine mapping of QTLs related to Pn, Tr, Ci, and Sc under the cold stress condition through the marker-assisted selection approaches.

中文翻译:

多环境低温胁迫下水稻光合作用相关性状的QTL分析

冷胁迫是影响水稻光合作用的主要非生物胁迫因素之一。在本研究中,水稻灌浆期光合作用相关性状(PRT)的连锁分析和全基因组关联研究(GWAS),包括净光合速率(Pn)、气孔导度(Sc)、蒸腾速率( Tr) 和细胞间 CO 2 浓度 (Ci) 是在冷胁迫下使用重组自交系群体和 295 个粳稻品种的单核苷酸多态性 (SNP) 重测序数据进行的。检测到 29 个加性 QTL(A-QTL),29 个 QTL 中有 17 个与冷胁迫相关。qPn7-1、qTr1、qTr7-1、qTr7-2、qCi7-1和qSc7-1在不同年份冷胁迫和控制条件下特异性表达,检测到一个主要的多效性 QTL 区间(RM1306-RM70)。此外,加性×环境互作QTL显示qPn7-1、qTr1、qTr7-1、qCi7-1、qSc7-1和qSc8-1主要受遗传因素影响,表明它们可用于耐寒育种活动。基于GWAS,10个SNP与冷水胁迫下的PRT显着相关,10个SNP中有3个包含在RM1306-RM70的主要QTL区间内,通过连锁分析验证了在多个环境中检测到的QTL。根据与 PRT 相关的主要 QTL 基因型,表型优越的 RIL47 可以考虑用于冷水胁迫下 PRT 的遗传改良。结果可促进与 Pn、Tr、Ci、
更新日期:2020-06-29
down
wechat
bug