In this research, new DABCO-based ionic liquid-modified Fe3O4 nanoparticles (NPs) were synthesized by the quaternization reaction. Modified magnetic NPs were characterized by different techniques. The functionalized magnetic NPs were utilized as a novel sorbent for magnetic SPE of imatinib mesylate (IMA) from human plasma samples followed with UPLC–MS–MS. Effective parameters on the extraction of IMA were studied by three-level Box–Behnken experimental design under response surface methodology. Under the optimized conditions, the proposed method was validated based on the principles of Guidance for Industry Bioanalytical Method Validation by the U.S. Food and Drug Administration. The linearity of the developed method was achieved within the range of 0.7–2500 ng mL−1. The LOD and lower limit of quantification were 0.68 and 0.21 ng mL−1, respectively. Satisfactory extraction recovery values ranged from 93 to 102% for human plasma samples with relative standard deviation values lower than 5.9%. The proposed analysis method was sensitive, efficient, rapid, and practical for the determination of IMA in biological samples.

中文翻译：

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使用基于 DABCO 的离子液体修饰磁性纳米颗粒灵敏测定人血浆中的甲磺酸伊马替尼

在这项研究中，通过季铵化反应合成了新的基于 DABCO 的离子液体改性的 Fe3O4 纳米粒子 (NPs)。改性磁性纳米颗粒通过不同的技术进行表征。功能化的磁性纳米颗粒被用作一种新型吸附剂，用于人血浆样品中甲磺酸伊马替尼 (IMA) 的磁性 SPE，然后进行 UPLC-MS-MS。在响应面方法下，通过三级 Box-Behnken 实验设计研究了 IMA 提取的有效参数。在优化的条件下，根据美国食品和药物管理局的工业生物分析方法验证指南的原则对所提出的方法进行了验证。所开发方法的线性度在 0.7–2500 ng mL-1 范围内。LOD 和定量下限分别为 0.68 和 0.21 ng mL-1，分别。人血浆样品的令人满意的提取回收率值范围为 93% 至 102%，相对标准偏差值低于 5.9%。所提出的分析方法灵敏、高效、快速、实用，可用于测定生物样品中的 IMA。