Oxidative stress can cause various diseases including inflammation, neurological disorders, cancer, diabetes, and cardiovascular diseases. Due to the current search for natural antioxidants, probiotics have received increasing scientific interest and are facing a growing industrial demand. Although various strains of lactobacilli and bifidobacteria are currently used in numerous health food supplements, their antioxidative activities have been relatively poorly identified. Therefore, in this work, we evaluated the in vitro effect of antioxidative activities (through assays of 2,2-diphenyl-l-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazorine-6-sulfonate) (ABTS) radical scavenging) and probiotic functional properties (cell viability in a simulated gastrointestinal tract, enzyme production, carbohydrate availability, and safely assessments) of Lactobacillus spp. and Bifidobacterium spp. isolated from human origins. From the nitric oxide (NO) assay screening, four strains (Bifidobacterium animalis subsp. lactis MG741, B. breve MG729, L. reuteri MG505, and L. rhamnosus MG316) were selected based on the yield amount of ferment productivity (> × 10¹⁰ CFU/g) and showed high antioxidant activities ranging from 22.2% to 38.2% in DPPH free radical scavenging, and 50.0% to 93.6% in ABTS radical scavenging. Regarding their functional properties as probiotics, these four strains were resistant to simulated gastric (pH 3 and 4) and intestinal fluids (pH 7 and 8), and showed potential for the promotion of health based on hemolysis, auto-aggregation, antibiotic susceptibility, enzyme production, and biochemical profiles. Altogether, our results showed that the selected probiotic strains may be good candidates as food ingredients to mitigate oxidative stress-related symptoms.

氧化应激会导致各种疾病，包括炎症，神经系统疾病，癌症，糖尿病和心血管疾病。由于目前正在寻找天然抗氧化剂，因此益生菌受到了越来越多的科学兴趣，并且面临着日益增长的工业需求。尽管目前在许多保健食品补品中使用了多种乳酸菌和双歧杆菌菌株，但它们的抗氧化活性却相对较差。因此，在这项工作中，我们评估了体外活性（通过2,2-二苯基-1-picylhydrazyl（DPPH）和2,2'-azinobis-（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）自由基清除的测定）和益生菌功能特性（细胞活力）在模拟胃肠道中，乳酸菌的酶产生，碳水化合物的利用和安全评估）。和双歧杆菌属。与人类起源隔离。从一氧化氮（NO）分析筛选中，根据发酵生产力的产量（>×10）选择了四个菌株（动物双歧杆菌乳酸亚种MG741，短双歧杆菌MG729，罗伊氏乳杆菌MG505和鼠李糖乳杆菌MG316）。¹⁰CFU / g），在DPPH自由基清除中表现出较高的抗氧化活性，范围为22.2％至38.2％，在ABTS自由基清除中显示为50.0％至93.6％。就其作为益生菌的功能特性而言，这四个菌株均对模拟胃（pH 3和4）和肠液（pH 7和8）具有抵抗力，并基于溶血，自动聚集，抗生素敏感性，酶的产生和生化特征。总之，我们的结果表明，所选的益生菌菌株可以作为缓解氧化应激相关症状的食品成分的良好候选者。